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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1993-8-5
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pubmed:abstractText |
Monoclonal antibodies recognizing the stable imidazole ring-opened form of the major N7-guanine aflatoxin B1-DNA adduct have been used in competitive enzyme-linked immunosorbent assays (ELISA) and indirect immunofluorescence assays to quantitate adduct levels in liver tissue. Methods were developed in AFB1-treated animals, then applied to paired tumor and nontumor liver tissues of hepatocellular carcinoma patients from Taiwan. An avidin-biotin complex staining method was also used for of the detection of hepatitis B surface (HBsAg) and X (HBxAg) antigens in liver sections. A total of 8 (30%) hepatocellular carcinoma (HCC) samples and 7 (26%) adjacent nontumor liver tissue samples from Taiwan were positive for AFB1-DNA adducts. For HBsAg, 10 (37%) HCC samples and 22 (81%) adjacent nontumorous liver samples were positive, and 9 (33%) HCC samples and 11 (41%) adjacent nontumor liver samples were HBxAg positive. No association with AFB1-DNA adducts was observed for HBsAg and HBxAg. These methods should be useful in determining the role of exposure in the induction of HCC in Taiwan.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0091-6765
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
199-202
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8391434-Aflatoxin B1,
pubmed-meshheading:8391434-Animals,
pubmed-meshheading:8391434-Carcinoma, Hepatocellular,
pubmed-meshheading:8391434-DNA,
pubmed-meshheading:8391434-DNA Adducts,
pubmed-meshheading:8391434-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8391434-Fluorescent Antibody Technique,
pubmed-meshheading:8391434-Hepatitis B Antigens,
pubmed-meshheading:8391434-Hepatitis B Surface Antigens,
pubmed-meshheading:8391434-Humans,
pubmed-meshheading:8391434-Immunohistochemistry,
pubmed-meshheading:8391434-Liver,
pubmed-meshheading:8391434-Liver Neoplasms,
pubmed-meshheading:8391434-Rats,
pubmed-meshheading:8391434-Trans-Activators
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pubmed:year |
1993
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pubmed:articleTitle |
Immunohistochemical detection of aflatoxin B1-DNA adducts and hepatitis B virus antigens in hepatocellular carcinoma and nontumorous liver tissue.
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pubmed:affiliation |
Comprehensive Cancer Center, School of Public Health, Columbia University, New York, NY 10032.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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