Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
13
|
| pubmed:dateCreated |
1993-7-30
|
| pubmed:abstractText |
To determine the T-cell receptor (TCR) V alpha/V beta gene usage of the human autologous gastric tumor-specific cytotoxic T-lymphocytes (CTLs), we first established two pairs of tumor cell lines, HST2 and SSTW, from the malignant peritoneal effusions of signet ring cell carcinomas and their peripheral blood lymphocyte-derived tumor-specific CD8-positive CTL lines, TcHST2 and TcSSTW. TCR V alpha/V beta gene usage from these CTL was examined using the reversely transcribed-polymerase chain reaction method, demonstrating that the V alpha 7, V alpha 12, and V beta 20 transcripts were commonly detected. The fact that repeated antigenic stimulation by mixed lymphocyte-autologous tumor cell cultures brought about the specific cytolysis and the restricted TCR usage of TCR V alpha 7, V alpha 12, and V beta 20 strongly suggests that these TCR V region products participated in T-cell-cancer interaction. This restricted TCR V gene usage in the gastric signet ring cell carcinomas led us to examine further the frequency of TCR V alpha/V beta usage in 11 cases of in vivo tumor-infiltrating lymphocytes with this particular type of tumor. The data showed that V alpha 7, V alpha 12, V beta 6, and V beta 20 were also predominantly expressed among these tumor-infiltrating lymphocytes in vivo. However, it seemed that T-cells with these TCR V region products are not specific for the gastric signet ring cell carcinomas, since they also frequently infiltrate into noncancerous lesions, such as peptic ulcers. These data may suggest that T-cells with certain TCR V alpha/V beta products could preferentially infiltrate into the stomach tissue, while some of these T-cells may be cytotoxic to the neoplastic autologous tumor cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0008-5472
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
53
|
| pubmed:geneSymbol |
TCR&agr;,
TCR&bgr;,
V&agr; 12,
V&agr; 7,
V&agr;/V&bgr;,
V&bgr; 20,
V&bgr; 6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3078-84
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8391386-Adenocarcinoma, Mucinous,
pubmed-meshheading:8391386-Base Sequence,
pubmed-meshheading:8391386-Duodenal Ulcer,
pubmed-meshheading:8391386-Humans,
pubmed-meshheading:8391386-Immunoglobulin Variable Region,
pubmed-meshheading:8391386-Immunotherapy,
pubmed-meshheading:8391386-Lymphocytes,
pubmed-meshheading:8391386-Lymphocytes, Tumor-Infiltrating,
pubmed-meshheading:8391386-Male,
pubmed-meshheading:8391386-Middle Aged,
pubmed-meshheading:8391386-Molecular Sequence Data,
pubmed-meshheading:8391386-Polymerase Chain Reaction,
pubmed-meshheading:8391386-Receptors, Antigen, T-Cell,
pubmed-meshheading:8391386-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:8391386-Stomach Neoplasms,
pubmed-meshheading:8391386-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:8391386-Transcription, Genetic,
pubmed-meshheading:8391386-Tumor Cells, Cultured
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Analysis of T-cell receptor V region gene usage of cytotoxic T-lymphocytes and tumor-infiltrating lymphocytes derived from human autologous gastric signet ring cell carcinomas.
|
| pubmed:affiliation |
Department of Pathology, Sapporo Medical University, School of Medicine, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|