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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1993-7-30
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pubmed:abstractText |
We established a vindesine-resistant (x 11.6) human small-cell lung cancer cell line (H69/VDS) by stepwise exposure of parent line H69 to vindesine. H69/VDS showed cross-resistance to taxol (x 10.1), vincristine (x 6.9) and colchicine (x 3.4) but not to doxorubicin, cisplatin or etoposide. There was no significant difference in intracellular [3H]-vincristine and doxorubicin accumulation between H69 and H69/VDS cells. The human mdr1 mRNA was not detected in either of the cell lines. These results indicated that H69/VDS did not express a typical multidrug resistant phenotype. Addition of 20 microM verapamil enhanced the growth inhibitory effect of vindesine on both H69/VDS (x 12.0) and H69 cells (x 3.8). The amount of total tubulin in H69/VDS cells was lower than that in the H69 parental cells. No significant increase was observed in the amount of total and polymerised tubulins of H69 cells. In H69/VDS cells, however, verapamil increased the amount of total tubulin to the level of parental cells, but decreased the amount of polymerised tubulin. Modulation of tubulin may play a role in the resistance to vindesine.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-1309222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-1419602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-1671676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-1972632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-1976450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-1995712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-2009540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-2164445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-2569930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-2746668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-2861892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-2888464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-3091555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-3459187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-423966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-6103535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-6113842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-6517872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391305-6606682
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0007-0920
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
74-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8391305-Antineoplastic Agents,
pubmed-meshheading:8391305-Blotting, Northern,
pubmed-meshheading:8391305-Blotting, Western,
pubmed-meshheading:8391305-Carcinoma, Small Cell,
pubmed-meshheading:8391305-Cell Division,
pubmed-meshheading:8391305-Clone Cells,
pubmed-meshheading:8391305-Drug Resistance,
pubmed-meshheading:8391305-Humans,
pubmed-meshheading:8391305-Kinetics,
pubmed-meshheading:8391305-Lung Neoplasms,
pubmed-meshheading:8391305-RNA, Neoplasm,
pubmed-meshheading:8391305-Tubulin,
pubmed-meshheading:8391305-Tumor Cells, Cultured,
pubmed-meshheading:8391305-Verapamil,
pubmed-meshheading:8391305-Vindesine
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pubmed:year |
1993
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pubmed:articleTitle |
Characterisation of a vindesine-resistant human small-cell lung cancer cell line.
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pubmed:affiliation |
Pharmacology Division of National Cancer Center Research Institute, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study
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