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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1993-7-30
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pubmed:abstractText |
We have investigated ubiquitinated paired helical filaments, which produce a proteinaceous smear in SDS-polyacrylamide gel electrophoresis and immunoblotting. The smear consisted largely of the carboxy-terminal portion of tau and ubiquitin. The ubiquitin-targeted protein was identified as tau in paired helical filaments, and the conjugation sites were localized to the microtubule-binding region. Most ubiquitin in paired helical filaments occurred as a monoubiquitinated form, and only a small proportion of ubiquitin formed multiubiquitin chains. There was a ubiquitin-negative smear, in which tau was much less processed in the amino-terminal portion. This strongly suggests that the amino-terminal processing of tau in paired helical filaments precedes its ubiquitination.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0896-6273
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1151-60
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8391280-Amino Acid Sequence,
pubmed-meshheading:8391280-Antibodies, Monoclonal,
pubmed-meshheading:8391280-Chromatography, High Pressure Liquid,
pubmed-meshheading:8391280-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8391280-Endopeptidases,
pubmed-meshheading:8391280-Humans,
pubmed-meshheading:8391280-Mass Spectrometry,
pubmed-meshheading:8391280-Microtubules,
pubmed-meshheading:8391280-Molecular Sequence Data,
pubmed-meshheading:8391280-Molecular Weight,
pubmed-meshheading:8391280-Neurofibrillary Tangles,
pubmed-meshheading:8391280-Peptide Fragments,
pubmed-meshheading:8391280-Peptide Mapping,
pubmed-meshheading:8391280-Protein Binding,
pubmed-meshheading:8391280-Ubiquitins,
pubmed-meshheading:8391280-tau Proteins
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pubmed:year |
1993
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pubmed:articleTitle |
Ubiquitin is conjugated with amino-terminally processed tau in paired helical filaments.
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pubmed:affiliation |
Department of Neuropathology, Faculty of Medicine, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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