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rdf:type |
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lifeskim:mentions |
umls-concept:C0004561,
umls-concept:C0017262,
umls-concept:C0021037,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0449774,
umls-concept:C0699040,
umls-concept:C1171362,
umls-concept:C1512666,
umls-concept:C1515670,
umls-concept:C1516356,
umls-concept:C2700116,
umls-concept:C2911684
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pubmed:issue |
1
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pubmed:dateCreated |
1993-7-23
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pubmed:abstractText |
Immunoglobulin gene rearrangement during mammalian B cell development generally follows an ordered progression, beginning with heavy (H) chain genes and proceeding through kappa and lambda light (L) chain genes. To determine whether the predicted kappa-->lambda hierarchy was occurring in vitro, we generated Epstein-Barr virus-transformed cell lines from cultures undergoing human pre-B cell differentiation. A total of 143 cell lines were established. 24 expressed cell surface mu/lambda by flow cytometry and were clonal by Southern blotting. Surprisingly, two of the mu/lambda-expressing cell lines contained both kappa alleles in germline configuration, and synthesis/expression of conventional lambda L chains was directly proven by immunoprecipitation/sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in one of them. Thus, human fetal bone marrow B lineage cells harbor the capacity to make functional lambda L chain gene rearrangements without rearranging or deleting either kappa allele. A third unusual cell line, designated 30.30, was observed to coexpress cell surface kappa and lambda L chains associated with mu H chains. The 30.30 cell line had a diploid karyotype, a single H chain rearrangement, both kappa alleles rearranged, and a single lambda rearrangement. Immunoprecipitation/SDS-PAGE confirmed that 30.30 cells synthesized and expressed kappa and lambda L chains. Multiparameter flow cytometry was used to demonstrate the existence of kappa+/lambda+ cells in fetal bone marrow and fetal spleen at frequencies of 2-3% of the total surface Ig+ B cell population. The flow cytometry data was confirmed by two-color immunofluorescence microscopy. The existence of normal human B cells expressing cell surface kappa and lambda refutes the widely accepted concept that expression of a single L chain isotype is immutable. The kappa+/lambda+ cells may represent transients undergoing L chain isotype switching.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-1108568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-14169351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-1590991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-1679470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-1719075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-1884018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-1906177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-1909409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-1911542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-1913803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-2115572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-2141629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-2188660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-2202665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-240887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-2411762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-2518725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-2538839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-2653368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-2694945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-2829207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-2961806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-2991929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-3035056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-3081897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-3126251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-3139764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-3927169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-405992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-4141694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-4158329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-6101209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-6273747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-6273911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-6300689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-6422305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-6775818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-6783958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-6791496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-6801526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8391059-6818320
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
178
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
139-49
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8391059-B-Lymphocytes,
pubmed-meshheading:8391059-Cell Differentiation,
pubmed-meshheading:8391059-Cell Line, Transformed,
pubmed-meshheading:8391059-Female,
pubmed-meshheading:8391059-Gene Rearrangement,
pubmed-meshheading:8391059-Genes, Immunoglobulin,
pubmed-meshheading:8391059-Hematopoietic Stem Cells,
pubmed-meshheading:8391059-Herpesvirus 4, Human,
pubmed-meshheading:8391059-Humans,
pubmed-meshheading:8391059-Immunoglobulin kappa-Chains,
pubmed-meshheading:8391059-Immunoglobulin lambda-Chains,
pubmed-meshheading:8391059-Pregnancy,
pubmed-meshheading:8391059-Receptors, Antigen, B-Cell
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pubmed:year |
1993
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pubmed:articleTitle |
Unusual patterns of immunoglobulin gene rearrangement and expression during human B cell ontogeny: human B cells can simultaneously express cell surface kappa and lambda light chains.
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pubmed:affiliation |
Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis 55455.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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