Linked Life Data

8390816

Source:http://linkedlifedata.com/resource/pubmed/id/8390816

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1993-7-21
pubmed:abstractText
Trichoderma reesei RUT C-30 formed an extracellular alpha-galactosidase when it was grown in a batch culture containing lactose or locust bean gum as a carbon source. Short-chain alpha-galactosides (melibiose, raffinose, stachyose), as well as the monosaccharides galactose, dulcitol, arabinose, and arabitol, also induced alpha-galactosidase activity both when they were used as carbon sources (at a concentration of 1%) in batch cultures and in resting mycelia (at concentrations in the millimolar range). The addition of 50 mM glucose did not affect the induction of alpha-galactosidase formation by galactose. alpha-Galactosidase from T. reesei RUT C-30 was purified to homogeneity from culture fluids of galactose-induced mycelia. The active enzyme was a 50 +/- 3-kDa, nonglycosylated monomer which had an isoelectric point of 5.2. It was active against several alpha-galactosides (p-nitrophenyl-alpha-D-galactoside, melibiose, raffinose, and stachyose) and galactomannan (locust bean gum) and was inhibited by the product galactose. It released galactose from locust bean gum and exhibited synergism with T. reesei beta-mannanase. Its activity was optimal at pH 4, and it displayed broad pH stability (pH 4 to 8). Its temperature stability was moderate (60 min at 50 degrees C resulted in recovery of 70% of activity), and its highest level of activity occurred at 60 degrees C. Its action on galactomannan was increased by the presence of beta-mannanase.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8390816-14323029, http://linkedlifedata.com/resource/pubmed/commentcorrection/8390816-2136347, http://linkedlifedata.com/resource/pubmed/commentcorrection/8390816-2287609, http://linkedlifedata.com/resource/pubmed/commentcorrection/8390816-39061, http://linkedlifedata.com/resource/pubmed/commentcorrection/8390816-4561015, http://linkedlifedata.com/resource/pubmed/commentcorrection/8390816-5418105, http://linkedlifedata.com/resource/pubmed/commentcorrection/8390816-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/8390816-5772469, http://linkedlifedata.com/resource/pubmed/commentcorrection/8390816-6331399, http://linkedlifedata.com/resource/pubmed/commentcorrection/8390816-782186, http://linkedlifedata.com/resource/pubmed/commentcorrection/8390816-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1347-53
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Conditions of formation, purification, and characterization of an alpha-galactosidase of Trichoderma reesei RUT C-30.
pubmed:affiliation
Abteilung für Mikrobielle Biochemie, Technische Universität Wien, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't