Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1993-7-12
pubmed:abstractText
The effects of formalin on the infectivity and immunogenicity of vesicular stomatitis virus (VSV) serotype Indiana were investigated. We found that formalin inactivation of VSV prevents infection of Vero cells in a concentration- and time-dependent manner, as shown by fluorometric cell analysis and inhibition of plaque formation. Inactivated VSV failed to induce significant cytotoxic T-lymphocyte responses in vivo or after restimulation in vitro. In contrast, the early immunoglobulin M (IgM) response, which is T help independent in the VSV system, was unaltered, suggesting normal antigenicity for and induction of B cells. However, no switch to IgG occurred, demonstrating failure of induction of T help. If cross-reactive T help was provided by previous infection with a second serotype of VSV (New Jersey), the IgG response was almost completely restored, confirming that the absence of IgG was due to lack of T help. A formalin-treated preparation of glycoprotein of VSV led to a delayed but otherwise normal IgG response, whereas treatment of VSV with UV light or beta-propiolactone reduced IgG titers to the same extent as did formalin. These results suggest that loss of infectivity and the ensuing lack of amplification of viral antigens of formaldehyde-inactivated VSV is the major factor impairing induction of specific T-helper cell responses.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-13641571, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-13926077, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-1658379, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-1692084, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-1700303, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-1710246, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-1710570, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-1741053, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-2176607, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-2435794, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-2849570, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-2981435, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-2982959, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-2986004, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-3007610, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-3022003, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-3029445, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-320661, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-3485173, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-3489186, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-399363, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-4118869, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-4190338, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-6154097, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-6180550, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-6180551, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-6288809, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-685887, http://linkedlifedata.com/resource/pubmed/commentcorrection/8389912-7684471
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3917-22
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Formalin inactivation of vesicular stomatitis virus impairs T-cell- but not T-help-independent B-cell responses.
pubmed:affiliation
Department of Pathology, University of Zürich, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't