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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1993-7-7
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pubmed:abstractText |
Protamines are small arginine-rich proteins that package DNA in spermatozoa. The mouse protamine 1 (Prm-1) gene is transcribed exclusively in post-meiotic spermatids. To identify elements in the Prm-1 promoter required for spermatid-specific transcription, we generated transgenic mice by microinjection of transgenes containing Prm-1 5' flanking sequences with 5' truncations or internal deletions of conserved sequences linked to a marked Prm-1 gene. We also tested Prm-1 promoter regions with a heterologous human growth hormone reporter gene. We conclude that a 113-bp region can direct spermatid-specific transcription and we have defined sequences within this region that are essential for proper function. These results will facilitate the isolation and characterization of transcription factors essential for post-meiotic gene expression.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-1537824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-1651914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-2043729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-2081589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-2300581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-2707490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-2740236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-2744460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-2744463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-2745455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-2747639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-2774416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-2885756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-3037541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-3070865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-3335501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-3358932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-3380096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-3382664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-3460029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-3600644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-3670302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-3683390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-3693351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-3754219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-3892534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-6198133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-6323022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-6468765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-6688229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-6692853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8389466-7096440
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
90
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pubmed:geneSymbol |
Prm-1,
Prm-2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5071-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8389466-Animals,
pubmed-meshheading:8389466-Base Sequence,
pubmed-meshheading:8389466-Blotting, Northern,
pubmed-meshheading:8389466-Growth Hormone,
pubmed-meshheading:8389466-Humans,
pubmed-meshheading:8389466-Male,
pubmed-meshheading:8389466-Mice,
pubmed-meshheading:8389466-Mice, Transgenic,
pubmed-meshheading:8389466-Molecular Sequence Data,
pubmed-meshheading:8389466-Mutagenesis, Site-Directed,
pubmed-meshheading:8389466-Promoter Regions, Genetic,
pubmed-meshheading:8389466-Protamines,
pubmed-meshheading:8389466-Sequence Deletion,
pubmed-meshheading:8389466-Simian virus 40,
pubmed-meshheading:8389466-Spermatids,
pubmed-meshheading:8389466-Testis
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pubmed:year |
1993
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pubmed:articleTitle |
Analysis of the mouse protamine 1 promoter in transgenic mice.
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pubmed:affiliation |
Howard Hughes Medical Institute, University of Washington, Seattle 98195.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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