Linked Life Data

8388995

Source:http://linkedlifedata.com/resource/pubmed/id/8388995

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1993-6-29
pubmed:abstractText
Uncoupling protein (UCP) gene expression is tightly restricted to thermogenic brown adipocytes and is rapidly activated by norepinephrine released after cold exposure. To identify cis-acting regulatory elements controlling this gene, a region encompassing 4.5 kilobases of DNA upstream of the transcription start site was analyzed using hybrid UCP-chloramphenicol acetyltransferase reporter gene constructs. Evidence for the presence of both tissue-specific and beta-adrenergic response elements in this 4.5-kilobase region was obtained by comparing the expression of these reporter genes in transfected brown adipocytes (in vitro differentiated), brown preadipocytes, white adipocytes, and Chinese hamster ovary (CHO) cells and from experiments in transgenic animals. Deletion analyses in transfected cells indicated that the minimal region exhibiting promoter activity and tissue specificity is located between -157 and -57 base pairs (bp). A 211-bp activator element located between -2494 and -2283 bp was necessary for full expression in brown adipocytes. This element also activated expression of the homologous -157-bp promoter and expression of a heterologous promoter in both brown adipocytes and CHO cells. A second region, downstream of the activator and possibly located between positions -400 and -157 bp, inhibited the UCP promoter in CHO cells. In mice transgenic for a chloramphenicol acetyltransferase reporter gene containing these elements, expression was both tissue specific and regulatable by environmental temperature changes. These results indicate that both positive and negative cis-acting elements participate in the regulation of UCP gene expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0888-8809
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
497-506
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8388995-Adipose Tissue, Brown, pubmed-meshheading:8388995-Animals, pubmed-meshheading:8388995-CHO Cells, pubmed-meshheading:8388995-Carrier Proteins, pubmed-meshheading:8388995-Cells, Cultured, pubmed-meshheading:8388995-Chloramphenicol O-Acetyltransferase, pubmed-meshheading:8388995-Cricetinae, pubmed-meshheading:8388995-Gene Deletion, pubmed-meshheading:8388995-Gene Expression Regulation, pubmed-meshheading:8388995-Ion Channels, pubmed-meshheading:8388995-Membrane Proteins, pubmed-meshheading:8388995-Mice, pubmed-meshheading:8388995-Mice, Transgenic, pubmed-meshheading:8388995-Mitochondrial Proteins, pubmed-meshheading:8388995-Norepinephrine, pubmed-meshheading:8388995-Promoter Regions, Genetic, pubmed-meshheading:8388995-Receptors, Adrenergic, beta, pubmed-meshheading:8388995-Recombinant Fusion Proteins, pubmed-meshheading:8388995-Simplexvirus, pubmed-meshheading:8388995-Thymidine Kinase, pubmed-meshheading:8388995-Transfection
pubmed:year
1993
pubmed:articleTitle
Tissue-specific and beta-adrenergic regulation of the mitochondrial uncoupling protein gene: control by cis-acting elements in the 5'-flanking region.
pubmed:affiliation
Centre de Recherche sur l'Endocrinologie Moléculaire et le Développement, Centre National de la Recherche Scientifique, Meudon, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't