Linked Life Data

8388992

Source:http://linkedlifedata.com/resource/pubmed/id/8388992

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1993-7-1
pubmed:abstractText
Bovine serum albumin greatly enhanced the cholinergic response mediated by neuronal nicotinic acetylcholine receptors in chick ciliary ganglion neurons. The enhancement exceeded 5-fold in some experiments (mean +/- standard error, 3.26 +/- 0.43-fold) and was rapid, was dose dependent, and occurred without changes in the unitary conductance or the mean open time of the acetylcholine receptor channel. This lack of detectable change in permeation or kinetic properties suggests that bovine serum albumin might increase acetylcholine responses by increasing the number of functional receptors. The enhancement appears to be specific to the albumin molecule, because activity could not be removed by detergent extraction, gel filtration, or dialysis. Acetylcholine responses in these cells are known to be enhanced by a cAMP-dependent mechanism that converts existing acetylcholine receptors from a nonfunctional to a functional state. We found that the enhancement by bovine serum albumin occurred without an increase in cAMP and that pretreatment with membrane-permeable cAMP analogs prevented any additional enhancement of the cholinergic response by bovine serum albumin. These observations are consistent with a cAMP-dependent modulation of the enhancement produced by bovine serum albumin or a convergence of the two enhancement mechanisms onto a single pathway.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0026-895X
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
807-12
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Modulation by albumin of neuronal cholinergic sensitivity.
pubmed:affiliation
Department of Pharmacology, University of California, San Diego, La Jolla 92093-0636.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't