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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5 Pt 1
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pubmed:dateCreated |
1993-6-22
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pubmed:abstractText |
Synthesis and secretion of surfactant protein A (SP-A) were studied in the isolated perfused rat lung using Trans35S-label (approximately 85% methionine, 15% cysteine) in the perfusate with or without 1 mM ATP or 0.1 mM 8-bromoadenosine 3',5',-cyclic monophosphate (8-BrcAMP) for up to 6 h of perfusion. By enzyme-linked immunosorbent assay, the SP-A content was 36 +/- 0.3% of total protein in extracellular surfactant and 10.8 +/- 1.9% of total protein in lamellar bodies of control lungs; these relativr proportions were maintained in the presence of ATP or 8-BrcAMP. Incorporation of [35S]methionine (cysteine) into the surfactant and lamellar body protein fraction could be detected at 4 h of perfusion. At 6 h, specific activity of total protein [disintegrations per minute (dpm)/micrograms)] was significantly increased in both the surfactant (54%) and lamellar body fractions (30%) under the influence of either secretagogue compared with control conditions. In the presence of ATP, there was a significant increase in the SP-A immunoprecipitable counts of 61 and 72% in extra- and intracellular compartments, respectively. However, no significant change was observed in the relative abundance of SP-A mRNA between control and secretagogue-treated lungs. This dissociation of SP-A mRNA abundance and label incorporation into protein indicates that alteration in translational efficiency or posttranslational factors may be involved in the secretagogue-induced stimulation of SP-A synthesis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/8-Bromo Cyclic Adenosine...,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Methionine,
http://linkedlifedata.com/resource/pubmed/chemical/Proteolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactants,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfur Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Tubulin
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0002-9513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
264
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
L431-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8388646-8-Bromo Cyclic Adenosine Monophosphate,
pubmed-meshheading:8388646-Adenosine Triphosphate,
pubmed-meshheading:8388646-Animals,
pubmed-meshheading:8388646-Autoradiography,
pubmed-meshheading:8388646-Blotting, Northern,
pubmed-meshheading:8388646-Blotting, Western,
pubmed-meshheading:8388646-Cyclic AMP,
pubmed-meshheading:8388646-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8388646-Lung,
pubmed-meshheading:8388646-Male,
pubmed-meshheading:8388646-Methionine,
pubmed-meshheading:8388646-Perfusion,
pubmed-meshheading:8388646-Proteolipids,
pubmed-meshheading:8388646-Pulmonary Surfactant-Associated Protein A,
pubmed-meshheading:8388646-Pulmonary Surfactant-Associated Proteins,
pubmed-meshheading:8388646-Pulmonary Surfactants,
pubmed-meshheading:8388646-RNA, Messenger,
pubmed-meshheading:8388646-Rats,
pubmed-meshheading:8388646-Rats, Sprague-Dawley,
pubmed-meshheading:8388646-Sulfur Radioisotopes,
pubmed-meshheading:8388646-Tubulin
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pubmed:year |
1993
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pubmed:articleTitle |
ATP and adenosine 3',5'-cyclic monophosphate stimulate the synthesis of surfactant protein A in rat lung.
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pubmed:affiliation |
Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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