Linked Life Data

8388226

Source:http://linkedlifedata.com/resource/pubmed/id/8388226

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1993-6-18
pubmed:abstractText
Amiloride has been suggested to inhibit responses to a variety of taste stimuli, including salty, sweet, and sour (acid). To test for the involvement of amiloride-sensitive Na+ channels in the transduction of acid stimuli, fungiform taste receptor cells were examined using patch-clamp techniques. Approximately one-half of all cells had amiloride-sensitive Na+ currents (INa) with a Ki value near 0.2 microM amiloride. After blocking voltage-gated conductances, cells having amiloride sensitivity were tested for responses to acid stimuli. Over three-fourths of cells showed an inward proton current (IH+) with an extrapolated reversal potential near approximately +150 mV, which was completely blocked by amiloride (30 microM). Treatment of isolated taste cells with arginine8-vasopressin caused equivalent increases in both INa and IH+; each effect was mimicked by 8-Br-cAMP. Taken together, these results indicate that protons permeate amiloride-sensitive Na+ channels in hamster fungiform taste cells and contribute to acid transduction.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0896-6273
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
931-42
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Proton currents through amiloride-sensitive Na+ channels in isolated hamster taste cells: enhancement by vasopressin and cAMP.
pubmed:affiliation
Department of Anatomy and Neurobiology, Colorado State University, Fort Collins 80523.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.