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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1993-6-4
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pubmed:abstractText |
The effect of parathyroid hormone (PTH) on acid/base transport in isolated rabbit renal proximal tubule S3 segment was investigated with double-barreled and conventional microelectrodes. PTH (10 nM) induced a small depolarization and enhanced the initial rates of cell pH (pHi) increase and cell Cl- ([Cl-]i) decrease in response to bath Cl- removal by 28.0 +/- 2.1% and 31.0 +/- 6.4% respectively. The calculated initial HCO3- influx to bath Cl- removal was also enhanced by 28%. On the other hand, PTH reduced the initial rate of pHi decrease to luminal Na+ removal in the absence of HCO3-/CO2 by 20.4 +/- 3.9%. The PTH-induced depolarization was not accompanied with changes in steady-state pHi or [Cl-]i levels, but was greatly attenuated in the presence of ouabain (0.1 mM). Either dibutyryl-cAMP (0.1 mM) plus theophylline (1 mM) or forskolin (10 microM) alone could reproduce all the effects of PTH. These results indicate that (a) PTH inhibits the luminal Na+/H+ exchanger but stimulates the basolateral Cl-/HCO3- exchanger in the S3 segment; (b) the PTH-induced depolarization largely results from inhibition of Na+/K(+)-ATPase and (c) all these effects are at least partly mediated by a cAMP-dependent mechanism.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bicarbonates,
http://linkedlifedata.com/resource/pubmed/chemical/Bucladesine,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chloride-Bicarbonate Antiporters,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Ouabain,
http://linkedlifedata.com/resource/pubmed/chemical/Parathyroid Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Hydrogen Antiporter,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Potassium-Exchanging ATPase,
http://linkedlifedata.com/resource/pubmed/chemical/Theophylline
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0031-6768
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
423
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7-13
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8387669-Animals,
pubmed-meshheading:8387669-Bicarbonates,
pubmed-meshheading:8387669-Biological Transport,
pubmed-meshheading:8387669-Bucladesine,
pubmed-meshheading:8387669-Carrier Proteins,
pubmed-meshheading:8387669-Chloride-Bicarbonate Antiporters,
pubmed-meshheading:8387669-Chlorides,
pubmed-meshheading:8387669-Cyclic AMP,
pubmed-meshheading:8387669-Female,
pubmed-meshheading:8387669-Forskolin,
pubmed-meshheading:8387669-Hydrogen-Ion Concentration,
pubmed-meshheading:8387669-Kidney Tubules, Proximal,
pubmed-meshheading:8387669-Membrane Potentials,
pubmed-meshheading:8387669-Ouabain,
pubmed-meshheading:8387669-Parathyroid Hormone,
pubmed-meshheading:8387669-Rabbits,
pubmed-meshheading:8387669-Sodium-Hydrogen Antiporter,
pubmed-meshheading:8387669-Sodium-Potassium-Exchanging ATPase,
pubmed-meshheading:8387669-Theophylline
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pubmed:year |
1993
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pubmed:articleTitle |
Effect of parathyroid hormone on acid/base transport in rabbit renal proximal tubule S3 segment.
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pubmed:affiliation |
First Department of Internal Medicine, Tokyo University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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