Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1993-6-1
pubmed:abstractText
Erythropoietin (Epo) synthesis increases in response to hypoxia. The hepatoma cell line Hep 3B produces low basal levels of Epo mRNA which increase markedly with hypoxia. To define the sequences necessary for this response, we linked fragments of the human Epo gene to a luciferase vector, introduced these plasmids into Hep 3B cells and assayed for luciferase activity after growth in 1% or 21% oxygen. A 621-bp Epo promoter fragment resulted in a 2.4-fold increase in luciferase activity with hypoxia. We tested several Epo gene fragments upstream of this Epo promoter fragment and found that a 613-bp Bgl II-Pvu II 3' fragment had a 10-fold increase in activity with hypoxia regardless of orientation. This fragment had a similar level of activity when linked to a simian virus 40 promoter. Portions of this fragment retained activity, including a 38-bp Apa I-Taq I fragment that had a 17-fold increase in activity with hypoxia. Deletion of nt 4-13 or 19-28 from this 38-bp fragment resulted in a loss of activity. The 24-bp upstream portion of the 38-bp fragment showed an 8-fold increase in activity with hypoxia. However, deletion of nt 19-24 or mutagenesis of nt 21 or 22 in this 24-bp fragment resulted in loss of activity. Our studies indicate that the transcriptional response of the human Epo gene to hypoxia is mediated in part by promoter sequences and to a greater degree by an enhancer element located in a 24-bp portion of the 3' flanking sequence of the gene.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-1448072, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-1448077, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-1584762, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-1874713, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-1885767, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-1940433, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-1961720, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-1985693, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-1985694, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-1991159, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-2062846, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-2123346, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-2384608, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-2546055, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-2644044, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-2825172, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-2849206, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-2910354, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-3009649, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-3032937, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-3337914, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-3593969, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-3773894, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-3821727, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-3881765, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-4637502, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-6346325, http://linkedlifedata.com/resource/pubmed/commentcorrection/8387202-6960240
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3928-32
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
A 24-base-pair sequence 3' to the human erythropoietin gene contains a hypoxia-responsive transcriptional enhancer.
pubmed:affiliation
Department of Pediatrics, University of California, San Francisco 94143-0724.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.