Linked Life Data

8386353

Source:http://linkedlifedata.com/resource/pubmed/id/8386353

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1993-5-18
pubmed:abstractText
The mechanism of enhancement of the L-type Ca current by a P2-purinergic agonist adenosine-5'-O-(3-thiotriphosphate) (ATP gamma S) was studied by recording single channel activity from cell-attached patches on rat isolated ventricular cells using patch pipettes containing 110 mM Ba2+. The application of ATP gamma S to the patch membrane through the pipette solution did not affect single channel activity. The addition of ATP gamma S to the bath containing a depolarizing solution was ineffective due to the voltage dependence of the purinergic stimulation. Bath application of ATP gamma S (100 microM) to control 4-(2-hydroxyethyl)-1-piperazine-ethanesulphonic acid (HEPES) solution increased the amplitude of ensemble average currents both by decreasing the probability of a blank sweep occurring and by increasing the number of openings per non-blank sweep. The single channel conductance (17 pS) was not changed by ATP gamma S. Both activation and inactivation curves were shifted towards hyperpolarized potentials by about 10 mV under P2-purinergic stimulation. Since ATP gamma S increased channel activity when applied via the bath, it must be supposed that a diffusible messenger is involved.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
422
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
465-71
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Modulation of L-type Ca channel activity by P2-purinergic agonist in cardiac cells.
pubmed:affiliation
U-241 INSERM, Physiologie Cellulaire Cardiaque, Université Paris-Sud, Orsay, France.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't