Linked Life Data

8386352

Source:http://linkedlifedata.com/resource/pubmed/id/8386352

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1993-5-18
pubmed:abstractText
Stilbenedisulfonic acid derivatives have been shown to block Cl- channels directly in many different preparations. Therefore, the utility of these compounds as tools for studying the cAMP-dependent Cl- current (ICl) in guinea-pig ventricular myocytes was examined using the patch-clamp technique to record whole-cell Cl- currents at room temperature. It was found that 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS) increased, rather than decreased, the isoproterenol (ISO)-activated Cl- current. However, SITS alone stimulated little or no sustained current, suggesting that SITS activates the Cl- current through a synergistic effect with ISO. 4,4'-Diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) also enhanced the ISO-activated Cl- current. However, 4,4'-dinitrostilbene-2,2'-disulfonic acid (DNDS) did not have any effect. SITS also exhibited a synergistic effect on the ISO-enhanced Ca2+ current in the same cells, suggesting that it affects the pathway involved in beta-adrenergic regulation of both Cl- and Ca2+ channels. SITS had no effect on the Cl- current stimulated by direct activation of adenylate cyclase with forskolin or exposure to the membrane-permeable cAMP derivative 8-bromoadenosine 3',5'-cyclic monophosphate. This suggests that SITS and DIDS may enhance the ISO-activated Cl- current via an effect on the beta-adrenergic receptor. It is concluded that these stilbenedisulfonic acid derivatives are not effective antagonists of cAMP-activated Cl- channels in cardiac ventricular myocytes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
422
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
436-42
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Effects of stilbenedisulfonic acid derivatives on the cAMP-regulated chloride current in cardiac myocytes.
pubmed:affiliation
Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH 44106-4970.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.