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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1993-5-17
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pubmed:abstractText |
Entry of herpes simplex virus (HSV) into cells is believed to be mediated by specific binding of envelope proteins to a cellular receptor. Neomycin specifically blocks this initial step in infection by HSV-1 but not HSV-2. Resistance of HSV-2 to this compound maps to a region of the genome encoding glycoprotein C (gC-2). We have studied the function of gC-2 in the initial interaction of the virus with the host cell, using HSV-2 mutants deleted for gC-2 and gC-2-rescued recombinants. Resistance to neomycin was directly linked to the presence of gC-2 within the viral genome. In addition, deletion of the gC-2 gene caused a marked delay in adsorption to cells relative to the wild-type virus. HSV-1 recombinants containing chimeric gC genes composed of HSV-1 and HSV-2 sequences were used to localize neomycin resistance within the N-terminal 223 amino acids of gC-2. This region of the glycoprotein comprises an important domain responsible for binding of HSV-2 to cell receptors in the presence of neomycin. A gC-2-negative mutant is still infectious, indicating that HSV-2 also has an alternative pathway of adsorption.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-1309916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-1310777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-1310996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-1322402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-1378512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-14468055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-1647025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-1649332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-1656593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-1847438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-1847442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2152816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2154609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2157859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2159526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2167550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-218224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-227992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2448875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2476575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2535752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2538243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2538244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2822948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2836609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2836952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2838567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2839594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2842148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2982036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-2991567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-3009845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-3009851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-3033823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-3036369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-4333719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-4348796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-6087328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-6092678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-6093367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8386261-6322160
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2434-41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8386261-Animals,
pubmed-meshheading:8386261-Base Sequence,
pubmed-meshheading:8386261-Chromosome Mapping,
pubmed-meshheading:8386261-Clone Cells,
pubmed-meshheading:8386261-Cricetinae,
pubmed-meshheading:8386261-DNA, Recombinant,
pubmed-meshheading:8386261-DNA, Viral,
pubmed-meshheading:8386261-Drug Resistance, Microbial,
pubmed-meshheading:8386261-Gene Deletion,
pubmed-meshheading:8386261-Molecular Sequence Data,
pubmed-meshheading:8386261-Mutation,
pubmed-meshheading:8386261-Neomycin,
pubmed-meshheading:8386261-Recombinant Fusion Proteins,
pubmed-meshheading:8386261-Recombination, Genetic,
pubmed-meshheading:8386261-Simplexvirus,
pubmed-meshheading:8386261-Viral Envelope Proteins,
pubmed-meshheading:8386261-Viral Proteins,
pubmed-meshheading:8386261-Virus Replication
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pubmed:year |
1993
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pubmed:articleTitle |
Resistance of herpes simplex virus type 2 to neomycin maps to the N-terminal portion of glycoprotein C.
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pubmed:affiliation |
Department of Microbiology and Immunology, Gade Institute, University of Bergen, Norway.
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pubmed:publicationType |
Journal Article
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