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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1993-5-10
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pubmed:abstractText |
A rapid and simple two-step scheme for the purification of herpes simplex virus type 1 UL8 protein from insect cells infected with a recombinant baculovirus has been developed. The scheme involves DEAE-Sepharose and phenyl-Sepharose chromatography and yields approximately 1.5 mg of protein from 2.4 x 10(8) infected cells. The protein remains intact during purification as judged by its reactivity with amino and carboxy termini-specific antisera. Gel filtration chromatography showed that the protein exists as a monomer in solution. No binding of the protein to ssDNA or dsDNA or to a DNA/RNA hybrid could be demonstrated using a gel mobility shift assay.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74 ( Pt 4)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
607-12
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8385691-Amino Acid Sequence,
pubmed-meshheading:8385691-Animals,
pubmed-meshheading:8385691-Baculoviridae,
pubmed-meshheading:8385691-Base Sequence,
pubmed-meshheading:8385691-Blotting, Western,
pubmed-meshheading:8385691-DNA Helicases,
pubmed-meshheading:8385691-DNA Primase,
pubmed-meshheading:8385691-Insects,
pubmed-meshheading:8385691-Macromolecular Substances,
pubmed-meshheading:8385691-Molecular Sequence Data,
pubmed-meshheading:8385691-Oligodeoxyribonucleotides,
pubmed-meshheading:8385691-RNA Nucleotidyltransferases,
pubmed-meshheading:8385691-Recombinant Proteins,
pubmed-meshheading:8385691-Simplexvirus,
pubmed-meshheading:8385691-Viral Proteins
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pubmed:year |
1993
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pubmed:articleTitle |
Purification and properties of the herpes simplex virus type 1 UL8 protein.
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pubmed:affiliation |
Medical Research Council Virology Unit, Institute of Virology, Glasgow, U.K.
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pubmed:publicationType |
Journal Article
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