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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5104
|
| pubmed:dateCreated |
1993-4-30
|
| pubmed:abstractText |
The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae. Vps34p shares significant sequence similarity with the catalytic subunit of bovine phosphatidylinositol (PI) 3-kinase [the 110-kilodalton (p110) subunit of PI 3-kinase], which is known to interact with activated cell surface receptor tyrosine kinases. Yeast strains deleted for the VPS34 gene or carrying vps34 point mutations lacked detectable PI 3-kinase activity and exhibited severe defects in vacuolar protein sorting. Overexpression of Vps34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p. VPS34 encodes a yeast PI 3-kinase, and this enzyme appears to regulate intracellular protein trafficking decisions.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0036-8075
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
2
|
| pubmed:volume |
260
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
88-91
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:8385367-Amino Acid Sequence,
pubmed-meshheading:8385367-Animals,
pubmed-meshheading:8385367-Brain,
pubmed-meshheading:8385367-Cattle,
pubmed-meshheading:8385367-Chromatography, High Pressure Liquid,
pubmed-meshheading:8385367-Fungal Proteins,
pubmed-meshheading:8385367-Gene Deletion,
pubmed-meshheading:8385367-Gene Expression,
pubmed-meshheading:8385367-Genes, Fungal,
pubmed-meshheading:8385367-Lysosomes,
pubmed-meshheading:8385367-Molecular Sequence Data,
pubmed-meshheading:8385367-Mutagenesis, Site-Directed,
pubmed-meshheading:8385367-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:8385367-Phosphotransferases,
pubmed-meshheading:8385367-Point Mutation,
pubmed-meshheading:8385367-Saccharomyces cerevisiae,
pubmed-meshheading:8385367-Sequence Homology, Amino Acid,
pubmed-meshheading:8385367-Signal Transduction,
pubmed-meshheading:8385367-Vacuoles
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting.
|
| pubmed:affiliation |
Division of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|