|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1993-4-23
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
Differentiated skeletal muscle cells cease dividing and sustain expression of a battery of tissue-specific genes. To identify regulators of growth and differentiation, we used a genetic complementation approach. Following introduction of a cDNA expression library into a differentiation-defective myoblast mutant (NMU2), cDNAs were isolated that activated muscle-specific promoters. The complementing cDNAs were identified as muscle structural genes, troponin I, tropomyosin, and alpha-cardiac actin, and their activity was mapped to the 3' untranslated region (3'UTR). The 3'UTRs augmented the differentiation of wild-type muscle cells. Upon expression in 10T1/2 fibroblasts, proliferation was suppressed, indicating that the effects of the 3'UTRs are not limited to myogenic cells. These data suggest that 3'UTRs of certain differentiation-specific RNAs are trans-acting regulators in a feedback loop that inhibits cell division and promotes differentiation.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Myog protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Myogenin,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tropomyosin,
http://linkedlifedata.com/resource/pubmed/chemical/Troponin,
http://linkedlifedata.com/resource/pubmed/chemical/Troponin I
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0092-8674
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
26
|
|
pubmed:volume |
72
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
903-17
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:8384533-Actins,
pubmed-meshheading:8384533-Animals,
pubmed-meshheading:8384533-Base Sequence,
pubmed-meshheading:8384533-Cell Differentiation,
pubmed-meshheading:8384533-Cell Division,
pubmed-meshheading:8384533-Cell Line,
pubmed-meshheading:8384533-DNA,
pubmed-meshheading:8384533-Gene Expression Regulation,
pubmed-meshheading:8384533-Genetic Complementation Test,
pubmed-meshheading:8384533-Mice,
pubmed-meshheading:8384533-Molecular Sequence Data,
pubmed-meshheading:8384533-Muscle Proteins,
pubmed-meshheading:8384533-Muscles,
pubmed-meshheading:8384533-Myogenin,
pubmed-meshheading:8384533-Oligodeoxyribonucleotides,
pubmed-meshheading:8384533-Promoter Regions, Genetic,
pubmed-meshheading:8384533-RNA, Messenger,
pubmed-meshheading:8384533-Tropomyosin,
pubmed-meshheading:8384533-Troponin,
pubmed-meshheading:8384533-Troponin I
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
Genetic complementation reveals a novel regulatory role for 3' untranslated regions in growth and differentiation.
|
|
pubmed:affiliation |
Department of Pharmacology, Stanford University School of Medicine, California 94305-5332.
|
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|
