Linked Life Data

8384482

Source:http://linkedlifedata.com/resource/pubmed/id/8384482

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1993-4-23
pubmed:abstractText
Protein kinase C (PKC) phosphorylated a synthetic peptide (CBP) that included the Thr-286 phosphorylation sequence and calmodulin binding domain of Ca2+/calmodulin-dependent protein kinase type II (CaM-kinase). Studies with a variety of truncated peptides suggested that the amino acid phosphorylated by PKC was Thr-286, the same amino acid that when autophosphorylated by Ca2+/calmodulin activation of CaM-kinase results in Ca2+/calmodulin-independent activity. These peptide studies also suggested that the C-terminal region of CBP is required to obtain maximal phosphorylation of Thr-286 by PKC. PKC also phosphorylated purified CaM-kinase from rat forebrain. Phosphopeptide analysis by one- and two-dimensional proteolytic maps of autophosphorylated CaM-kinase and CaM-kinase phosphorylated with PKC identified that there are both similar and unique sites phosphorylated. Phosphoamino acid analysis of CaM-kinase phosphorylated by PKC indicated that both Ser and Thr residues were phosphorylated. Even though Thr-286 of CaM-kinase appeared to be phosphorylated by PKC, no Ca2+/calmodulin-independent activity was detected, and, additionally, no significant change in Ca2+/CaM-dependent activation was detected. These results provide the first indication that these two important protein kinases may communicate directly through interenzyme phosphorylation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2923-30
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Ca2+/calmodulin-dependent protein kinase II is phosphorylated by protein kinase C in vitro.
pubmed:affiliation
Department of Neurobiology and Anatomy, University of Texas Health Science Center, Houston 77225.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.