Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-4-22
pubmed:abstractText
The RecE recombination pathway is active in Escherichia coli recB recC sbcA mutants. To isolate and characterize products and intermediates of RecE-mediated, break-induced, intramolecular recombination, we infected recB recC sbcA mutants, expressing EcoRI endonuclease, with chimeric lambda phages that allow EcoRI-mediated release of cloned linear recombination substrates. Substrates with direct terminal repeats recombined to yield a circular product with one copy of the repeated sequence. Some recombinants were heteroallelic for the recombining markers. Markers distant to the break were recovered in the circular product at a higher frequency than markers close to the break. To examine the heteroduplex structures that may have yielded the heteroallelic recombinants, nonreplicative substrates were employed. Some of the nonreplicative recombination products contained heteroduplexes, with a strong bias for paired strands ending 3' at the break. This strand bias in heteroduplex formation is consistent with recombination models that postulate homologous pairing of protruding 3' single-stranded ends.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-13475269, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-149110, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-1542666, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-1732167, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-1829428, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-1836442, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-2631699, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-2670665, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-2949853, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-2957271, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-2958632, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-2961561, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-3044922, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-3058984, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-4248156, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-4416166, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-4568763, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-4610579, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-4894690, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-4961270, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-6162401, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-6257642, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-6269464, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-6308623, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-6309804, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-6315951, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-6330525, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-776705, http://linkedlifedata.com/resource/pubmed/commentcorrection/8384141-940351
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
133
pubmed:geneSymbol
luxA, recB, recC, recE, sbcA
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
439-48
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Heteroduplex strand-specificity in restriction-stimulated recombination by the RecE pathway of Escherichia coli.
pubmed:affiliation
Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't