Linked Life Data

8383722

Source:http://linkedlifedata.com/resource/pubmed/id/8383722

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1993-4-12
pubmed:abstractText
The effects of the antimycobacterial agent clofazimine and its experimental analogue B669 on the phospholipase A2 activity of and superoxide generation by human blood neutrophils were investigated in vitro. Coincubation of neutrophils with clofazimine or B669 (1-10 micrograms/mL) was accompanied by a dose-related increase in the release of both [3H]lysophosphatidylcholine (LPC) and [3H]arachidonate (AA). Both agents (1 microgram/mL) also increased the production of superoxide by resting and FMLP-stimulated neutrophils. Addition of reagent AA and especially LPC to neutrophils mimicked the effects of the riminophenazines, while alpha-tocopherol, a lysophospholipid complex-forming agent, neutralized the prooxidative interactions of clofazimine and B669 with these cells. These observations demonstrate that LPC, and to a lesser extent AA, generated during exposure of human neutrophils to clofazimine or B669 are the primary mediators of the prooxidative interactions of these riminophenazines with phagocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-1899
pubmed:author
pubmed:issnType
Print
pubmed:volume
167
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
899-904
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
An in vitro comparison of the effects of the prooxidative riminophenazines clofazimine and B669 on neutrophil phospholipase A2 activity and superoxide generation.
pubmed:affiliation
Department of Hematology, Medical University of Southern Africa, Medunsa.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro