Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-3-26
pubmed:abstractText
As part of an investigation into the effects of endogenous retroviruses on adjacent genes, we have isolated a cDNA clone derived from the human teratocarcinoma cell line NTera2D1 representing a chimeric transcript in which an endogenous retrovirus-like element, RTVL-H, has been spliced to downstream cellular sequences. The 5' terminus of this clone, termed AF-5, occurs one bp downstream of the predicted transcriptional start site in the RTVL-H long terminal repeat (LTR). AF-5 contains an open reading frame of 689 amino acids beginning within RTVL-H sequences that has two domains of homology with phospholipase A2 (PLA2). These domains, of approximately 120 amino acids each, are 30-38% identical to secreted PLA2s and contain sequence features of both group I and II enzymes. The corresponding AF-5 transcript is 2.5 kb and is derived from a single copy novel gene termed PLA2L. Southern analysis indicates that the RTVL-H element is normally present in human DNA upstream of the PLA2L gene. RTVL-H/PLA2L chimeric transcripts were detected in two independent teratocarcinoma cell lines but not in several other cell lines or primary human tissues. Characterization of additional cDNA clones and PCR analysis indicates that multiple RTVL-H/PLA2L alternatively spliced transcripts are expressed. No evidence has been found for transcription from a non-LTR promoter. These findings strongly suggest that the endogenous LTR promotes expression of the human PLA2L gene in teratocarcinoma cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-1317316, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-1379564, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-1505956, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-1659665, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-1690875, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-1713126, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-1851374, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-1878124, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-1906605, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2115127, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2120459, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2167160, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2178239, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2325205, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2400792, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2413359, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2446010, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2454389, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2544084, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2551777, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2596030, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2606907, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2684548, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2698190, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2722967, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2775276, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2776767, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2884330, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2925608, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-2925633, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-3028739, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-3037490, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-3146900, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-3167981, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-3188064, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-3220471, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-3313277, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-3346101, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-3474623, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-3714490, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-3754861, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-6095301, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-6312838, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-6349696, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-6546423, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-6694356, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-7061414, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-7066985, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-7135414, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-7222082, http://linkedlifedata.com/resource/pubmed/commentcorrection/8382789-873920
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
21
pubmed:geneSymbol
PLA2L
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
135-43
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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