|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:dateCreated |
1993-3-23
|
|
pubmed:abstractText |
Experiments were carried out to determine whether or not CDP-diacylglycerol:myo-inositol 3-phosphatidyltransferase (IT) activity (EC 2.7.8.11) could be detected in purified plasma-membrane fractions from WRK-1 rat mammary tumour cells. These cells have previously been shown to have a very active phosphoinositide cycle. Sucrose-density-gradient-purified plasma membranes contained no IT activity that could not be accounted for by endoplasmic-reticulum contamination. However, we also determined that the relative amount of IT activity in endoplasmic reticulum and plasma-membrane fractions could be altered by changing the concentration of detergent in the assay system.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-100216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-1312094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-1657963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-1850237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-18992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-212440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-2156558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-2160284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-2173558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-2541501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-2820960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-2826485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-2840949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-2985595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-3029593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-3924909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-4138239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-4291255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-4371924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-4425464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-6330069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-6330070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8382477-7037762
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0264-6021
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
290 ( Pt 1)
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
179-83
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:8382477-Animals,
pubmed-meshheading:8382477-CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase,
pubmed-meshheading:8382477-Cell Fractionation,
pubmed-meshheading:8382477-Cell Membrane,
pubmed-meshheading:8382477-Centrifugation, Density Gradient,
pubmed-meshheading:8382477-Detergents,
pubmed-meshheading:8382477-Endoplasmic Reticulum,
pubmed-meshheading:8382477-Kinetics,
pubmed-meshheading:8382477-Mammary Neoplasms, Experimental,
pubmed-meshheading:8382477-Membrane Proteins,
pubmed-meshheading:8382477-Phosphatidylinositols,
pubmed-meshheading:8382477-Phosphotransferases,
pubmed-meshheading:8382477-Rats,
pubmed-meshheading:8382477-Subcellular Fractions,
pubmed-meshheading:8382477-Transferases (Other Substituted Phosphate Groups),
pubmed-meshheading:8382477-Tumor Cells, Cultured
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
Organization of the phosphoinositide cycle. Assessment of inositol transferase activity in purified plasma membranes.
|
|
pubmed:affiliation |
Department of Physiology & Biophysics, N.Y.U. Medical Center, New York.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.
|
