Linked Life Data

8382019

Source:http://linkedlifedata.com/resource/pubmed/id/8382019

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-3-16
pubmed:abstractText
A solid phase DNA sequencing method for non-radioactive detection of single base changes without the need for electrophoresis is presented. The concept relies on the detection of DNA polymerase activity by an enzymatic luminometric inorganic pyrophosphate detection assay (P. Nyrén, 1987, Anal. Biochem. 167, 235-238). Immobilized DNA fragments amplified with the polymerase chain reaction are used as template. A detection primer is annealed in front of the mutation and four aliquots of this mixture are incubated with DNA polymerase and one of the four different dideoxynucleotides. The presence or absence of an incorporated dideoxynucleotide is thereafter monitored by the release of inorganic pyrophosphate during the following primer extension step. We show that the concept can be used for sequencing of single bases as well as stepwise analysis of several subsequent bases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:volume
208
pubmed:geneSymbol
pol
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
171-5
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Solid phase DNA minisequencing by an enzymatic luminometric inorganic pyrophosphate detection assay.
pubmed:affiliation
Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't