Linked Life Data

8381074

Source:http://linkedlifedata.com/resource/pubmed/id/8381074

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-3-2
pubmed:databankReference
pubmed:abstractText
The transcriptional transactivational activities of the phosphoprotein cAMP-response element-binding protein (CREB) are activated by the cAMP-dependent protein kinase A signaling pathway. Dimers of CREB bind to the palindromic DNA element 5'-TGACGTCA-3' (or similar motifs) called cAMP-responsive enhancers (CREs) found in the control regions of many genes, and activate transcription in response to phosphorylation of CREB by protein kinase A. Earlier we reported on the cyclical expression of the CREB gene in the Sertoli cells of the rat testis that occurred concomitant with the FSH-induced rise in cellular cAMP levels and suggested that transcription of the CREB gene may be autoregulated by cAMP-dependent transcriptional proteins. We now report the structure of the 5'-flanking sequence of the human CREB gene containing promoter activity. The promoter has a high content of guanosines and cytosines and lacks canonical TATA and CCAAT boxes typically found in the promoters of genes in eukaryotes. Notably, the promoter contains three CREs and transcriptional activities of a promoter-luciferase reporter plasmid transfected to placental JEG-3 cells are increased 3- to 5-fold over basal activities in response to either cAMP or 12-O-tetradecanoyl phorbol-14-acetate, and give 6- to 7-fold responses when both agents are added. The CREs bind recombinant CREB and endogenous CREB or CREB-like proteins contained in placental JEG-3 cells and also confer cAMP-inducible transcriptional activation to a heterologous minimal promoter. Our studies suggest that the expression of the CREB gene is positively autoregulated in trans.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
132
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
770-80
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8381074-Amino Acid Sequence, pubmed-meshheading:8381074-Base Sequence, pubmed-meshheading:8381074-Cell Nucleus, pubmed-meshheading:8381074-Choriocarcinoma, pubmed-meshheading:8381074-Cloning, Molecular, pubmed-meshheading:8381074-Cyclic AMP, pubmed-meshheading:8381074-Cyclic AMP Response Element-Binding Protein, pubmed-meshheading:8381074-DNA, pubmed-meshheading:8381074-Exons, pubmed-meshheading:8381074-Female, pubmed-meshheading:8381074-Gene Expression Regulation, Neoplastic, pubmed-meshheading:8381074-Humans, pubmed-meshheading:8381074-Luciferases, pubmed-meshheading:8381074-Molecular Sequence Data, pubmed-meshheading:8381074-Nuclear Proteins, pubmed-meshheading:8381074-Oligodeoxyribonucleotides, pubmed-meshheading:8381074-Pregnancy, pubmed-meshheading:8381074-Promoter Regions, Genetic, pubmed-meshheading:8381074-Protein Biosynthesis, pubmed-meshheading:8381074-Protein Kinases, pubmed-meshheading:8381074-RNA, Neoplasm, pubmed-meshheading:8381074-Recombinant Fusion Proteins, pubmed-meshheading:8381074-Transcription, Genetic, pubmed-meshheading:8381074-Tumor Cells, Cultured, pubmed-meshheading:8381074-Uterine Neoplasms
pubmed:year
1993
pubmed:articleTitle
The promoter of the gene encoding 3',5'-cyclic adenosine monophosphate (cAMP) response element binding protein contains cAMP response elements: evidence for positive autoregulation of gene transcription.
pubmed:affiliation
Laboratory of Molecular Endocrinology, Massachusetts General Hospital, Boston 02114.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't