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rdf:type |
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lifeskim:mentions |
umls-concept:C0012888,
umls-concept:C0023043,
umls-concept:C0038975,
umls-concept:C0086418,
umls-concept:C0205369,
umls-concept:C0598312,
umls-concept:C1514562,
umls-concept:C1546857,
umls-concept:C1704675,
umls-concept:C1704686,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
2
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pubmed:dateCreated |
1993-2-24
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pubmed:abstractText |
Initiation of cell-free simian virus 40 (SV40) DNA replication requires the interaction of DNA polymerase alpha/primase with a preinitiation complex containing the viral T antigen and cellular proteins, replication protein A, and topoisomerase I or II. To further understand the molecular mechanisms of the transition from preinitiation to initiation, the intermolecular interaction between human DNA polymerase alpha and T antigen was investigated. We have demonstrated that the human DNA polymerase alpha catalytic polypeptide is able to associate with SV40 large T antigen directly under physiological conditions. A physical association between these two proteins was detected by coimmunoprecipitation with monoclonal antibodies from insect cells coinfected with recombinant baculoviruses. A domain of human polymerase alpha physically interacting with T antigen was identified within the amino-terminal region from residues 195 to 313. This domain of human polymerase alpha was able to form a nonproductive complex with T antigen, causing inhibition of the SV40 DNA replication in vitro. Kinetics of the inhibition indicated that this polymerase domain can inhibit viral replication only during the preinitiation stage. Extra molecules of T antigen could partially overcome the inhibition only prior to initiation complex formation. The data support the conclusion that initiation of SV40 DNA replication requires the physical interaction of T antigen in the preinitiation complex with the amino-terminal domain of human polymerase alpha from amino acid residues 195 to 313.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-1311258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-1579162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-1579168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-1671045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-1671046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-1698613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-1848671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-1902230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-1939281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-1970792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-1976634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2153460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2165567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2168968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2243771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2549858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2550797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2550804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2557059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2829177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2832743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2833742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2841119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2843291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2848017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2994044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-2995982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-3018548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-3025630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-3025851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-3031654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-3035543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-3055666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-3070325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-3311385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-3532863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-6095264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-6155477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-6169844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-6251547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380896-7085672
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
809-20
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8380896-Antigens, Viral, Tumor,
pubmed-meshheading:8380896-Base Sequence,
pubmed-meshheading:8380896-Binding Sites,
pubmed-meshheading:8380896-Catalysis,
pubmed-meshheading:8380896-DNA, Viral,
pubmed-meshheading:8380896-DNA Polymerase II,
pubmed-meshheading:8380896-DNA Replication,
pubmed-meshheading:8380896-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8380896-Humans,
pubmed-meshheading:8380896-Molecular Sequence Data,
pubmed-meshheading:8380896-Precipitin Tests,
pubmed-meshheading:8380896-Recombinant Fusion Proteins,
pubmed-meshheading:8380896-Simian virus 40
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pubmed:year |
1993
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pubmed:articleTitle |
Initiation of simian virus 40 DNA replication requires the interaction of a specific domain of human DNA polymerase alpha with large T antigen.
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pubmed:affiliation |
Department of Pathology, Stanford University School of Medicine, California 94305-5324.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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