Linked Life Data

8380658

Source:http://linkedlifedata.com/resource/pubmed/id/8380658

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-2-17
pubmed:abstractText
The C11A mutant of SV40 large T antigen is unable to support the replication of viral origin containing DNA (ori-DNA) in vivo or in vitro. The mutation within C11A at residue 522 (pro-->ser) is located within the presumptive ATPase region of T antigen. While C11A T antigen was previously reported to be defective in ATPase and DNA helicase activities, it was shown to be capable of binding specifically to DNA containing the viral replication origin. As the positions of many conditional mutations of SV40 T antigen are located within the ATPase domain we asked whether C11A might also exhibit temperature-sensitive defects. We found that several activities of C11A T antigen are conditionally defective. C11A T antigen was able to hydrolyze ATP, assemble into hexamers, and display ATP-dependent alterations in DNA binding and ori-DNA structure at 33 degrees but not 41 degrees. Wild-type T antigen did not exhibit temperature-sensitive defects in these activities. C11A T antigen was completely unable to unwind ori-DNA at either temperature. This defect in unwinding was trans-dominant; C11A T antigen inhibited ori-DNA unwinding by wild-type T antigen. These data show that a mutant displaying a nonconditional defective phenotype may contain a subset of relevant properties that are temperature sensitive.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
192
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
402-14
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Unusual properties of a replication-defective mutant SV40 large T antigen.
pubmed:affiliation
Department of Biological Sciences, Columbia University, New York, New York 10027.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.