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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1993-2-11
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pubmed:abstractText |
We have previously reported that the equine herpesvirus 1 (EHV-1) XbaI G restriction fragment (nucleotides 1436 to 7943 relative to the left terminus of the EHV-1 genome [Kentucky A strain]) is required in combination with the EHV-1 immediate-early (IE) gene to achieve significant activation of two representative EHV-1 late promoter-chloramphenicol acetyltransferase (CAT) recombinants in transient expression assays. In this report, we demonstrate that the XbaI G-encoded UL3 gene (an ICP27 homolog) provides a trans-acting factor which acts (in combination with the EHV-1 IE gene product) to increase reporter gene expression directed by an EHV-1 late promoter-CAT recombinant plasmid. We show that cloned copies of UL3 can successfully substitute for the XbaI G fragment in CAT assays and that stop codon insertion within the UL3 open reading frame inhibits the ability of UL3 to activate reporter gene expression in trans.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-1309283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-1309921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-1314054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-1315705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-1316680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-1318606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-1323700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-1329372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-1645901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-1649513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-1845821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2157053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2161048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2161950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2177089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2535723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2545913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2549711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2552143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2555546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2825444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2833023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2843677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2845657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2846867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2990102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-2991596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-3023536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-3033896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-6178795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-6244694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380457-6255207
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1105-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8380457-Codon,
pubmed-meshheading:8380457-Gene Expression Regulation, Viral,
pubmed-meshheading:8380457-Genes, Viral,
pubmed-meshheading:8380457-Herpesvirus 1, Equid,
pubmed-meshheading:8380457-Immediate-Early Proteins,
pubmed-meshheading:8380457-Promoter Regions, Genetic,
pubmed-meshheading:8380457-Reading Frames,
pubmed-meshheading:8380457-Recombinant Proteins,
pubmed-meshheading:8380457-Trans-Activators,
pubmed-meshheading:8380457-Transcriptional Activation,
pubmed-meshheading:8380457-Viral Proteins
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pubmed:year |
1993
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pubmed:articleTitle |
The equine herpesvirus 1 (EHV-1) UL3 gene, an ICP27 homolog, is necessary for full activation of gene expression directed by an EHV-1 late promoter.
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pubmed:affiliation |
Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130-3932.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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