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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0001480,
umls-concept:C0006100,
umls-concept:C0006675,
umls-concept:C0020200,
umls-concept:C0021549,
umls-concept:C0030685,
umls-concept:C0037817,
umls-concept:C0391871,
umls-concept:C0680255,
umls-concept:C0871261,
umls-concept:C1283071,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1963578,
umls-concept:C2828360,
umls-concept:C2911692
|
| pubmed:issue |
2
|
| pubmed:dateCreated |
1993-2-9
|
| pubmed:abstractText |
Addition of endothelins (ETs) to neuroblastoma-glioma hybrid cells (NG108-15) induced increases in cytosolic free Ca2+ ([Ca2+]i) levels of labeled inositol monophosphates and inositol 1,4,5-trisphosphate [Ins(1,4,5)P3]. The increases in [Ca2+]i elicited by the three ETs (ET-1, ET-2, and ET-3) were transient and did not show a sustained phase. Chelating extracellular Ca2+ in the medium by adding excess EGTA decreased the ET-mediated Ca2+ response by 40-50%. This result indicates that a substantial portion of the increase in [Ca2+]i was due to influx from an extracellular source. However, the increase in [Ca2+]i was not affected by verapamil or nifedipine (10(-5) M). A rank order potency of ET-1 > ET-2 > ET-3 is shown for the stimulated increase in [Ca2+]i, as well as labeled inositol phosphates, in these cells. ATP (10(-4) M) and bradykinin (10(-7) M) also induced the increases in [Ca2+]i and Ins(1,4,5)P3 in NG108-15 cells, albeit to a different extent. When compared at 10(-7) M, bradykinin elicited a five- to sixfold higher increase in the level of Ins(1,4,5)P3, but less than a twofold higher increase in [Ca2+]i than those induced by ET-1. Additive increases in both Ins(1,4,5)P3 and [Ca2+]i were observed when ET-1, ATP, and bradykinin were added to the cells in different combinations, suggesting that each receptor agonist is responsible for the hydrolysis of a pool of polyphosphoinositide within the membrane. ET-1 exhibited homologous desensitization of the Ca2+ response, but partial heterologous desensitization to the Ca2+ response elicited by ATP. On the contrary, ET-1 did not desensitize the response elicited by bradykinin, although bradykinin exhibited complete heterologous desensitization to the response elicited by ET-1. Taken together, these results illustrate that, in NG108-15 cells, a considerable amount of receptor cross talk occurs between ET and other receptors that transmit signals through the polyphosphoinositide pathway.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Bradykinin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelins,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate...,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
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| pubmed:issn |
0022-3042
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
60
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
454-60
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| pubmed:dateRevised |
2007-7-18
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| pubmed:meshHeading |
pubmed-meshheading:8380432-Adenosine Triphosphate,
pubmed-meshheading:8380432-Animals,
pubmed-meshheading:8380432-Bradykinin,
pubmed-meshheading:8380432-Calcium,
pubmed-meshheading:8380432-Calcium Channels,
pubmed-meshheading:8380432-Egtazic Acid,
pubmed-meshheading:8380432-Endothelins,
pubmed-meshheading:8380432-Fura-2,
pubmed-meshheading:8380432-Glioma,
pubmed-meshheading:8380432-Hybrid Cells,
pubmed-meshheading:8380432-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:8380432-Inositol 1,4,5-Trisphosphate Receptors,
pubmed-meshheading:8380432-Kinetics,
pubmed-meshheading:8380432-Neuroblastoma,
pubmed-meshheading:8380432-Phosphatidylinositol Phosphates,
pubmed-meshheading:8380432-Phosphatidylinositols,
pubmed-meshheading:8380432-Radioligand Assay,
pubmed-meshheading:8380432-Receptors, Cell Surface,
pubmed-meshheading:8380432-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:8380432-Time Factors
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| pubmed:year |
1993
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| pubmed:articleTitle |
Endothelin-mediated calcium response and inositol 1,4,5-trisphosphate release in neuroblastoma-glioma hybrid cells (NG108-15): cross talk with ATP and bradykinin.
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| pubmed:affiliation |
Division of Cardiovascular Research, Academia Sinica, Nankang, Taipei, Taiwan, R.O.C.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|