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rdf:type |
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lifeskim:mentions |
umls-concept:C0008657,
umls-concept:C0017262,
umls-concept:C0023487,
umls-concept:C0030705,
umls-concept:C0152035,
umls-concept:C0185117,
umls-concept:C0439064,
umls-concept:C0475264,
umls-concept:C1519595,
umls-concept:C1826672,
umls-concept:C2003941,
umls-concept:C2603343,
umls-concept:C2911684
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pubmed:issue |
1
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pubmed:dateCreated |
1993-2-2
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pubmed:abstractText |
Translocation (15;17)(q22;q12-q21) is a chromosome aberration specifically found in acute promyelocytic leukemia (APL), that generates a chimeric gene between the promyelocytic leukemia (PML) gene on chromosome 15 and the retinoic acid receptor alpha (RARA) gene, on chromosome 17. In the course of molecular investigations of a series of 28 Chinese patients with APL, we have simultaneously used Southern blot and reverse transcriptase polymerase chain reaction (RT-PCR) analysis to characterize the PML gene breakpoints on chromosome 15 and identify PML-RARA fusion transcripts. Our results confirmed the existence of the three recently described bcr1, bcr2, and bcr3 breakpoint cluster regions. In addition, structural data provided by PML-RARA transcripts allowed us to more accurately locate the 3' borders of clusters bcr1 and bcr3. Moreover, our data suggest a preferential localization of the breakpoints within bcr1 and bcr3. The primary structure of a 1.4 kb DNA segment flanking the 5' part of the PML gene and that of the bcr3 cluster (2.1 kb) were also established.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BCR protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PML protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcr,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Retinoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0887-6924
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:geneSymbol |
PML,
RARA,
myl
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
20-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8380300-Base Sequence,
pubmed-meshheading:8380300-Carrier Proteins,
pubmed-meshheading:8380300-China,
pubmed-meshheading:8380300-Chromosomes, Human, Pair 15,
pubmed-meshheading:8380300-Chromosomes, Human, Pair 17,
pubmed-meshheading:8380300-DNA, Neoplasm,
pubmed-meshheading:8380300-Gene Expression,
pubmed-meshheading:8380300-Genes,
pubmed-meshheading:8380300-Humans,
pubmed-meshheading:8380300-Leukemia, Promyelocytic, Acute,
pubmed-meshheading:8380300-Molecular Sequence Data,
pubmed-meshheading:8380300-Neoplasm Proteins,
pubmed-meshheading:8380300-Nuclear Proteins,
pubmed-meshheading:8380300-Protein-Tyrosine Kinases,
pubmed-meshheading:8380300-Proto-Oncogene Proteins,
pubmed-meshheading:8380300-Proto-Oncogene Proteins c-bcr,
pubmed-meshheading:8380300-RNA, Messenger,
pubmed-meshheading:8380300-RNA, Neoplasm,
pubmed-meshheading:8380300-Receptors, Retinoic Acid,
pubmed-meshheading:8380300-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8380300-Restriction Mapping,
pubmed-meshheading:8380300-Transcription Factors,
pubmed-meshheading:8380300-Translocation, Genetic,
pubmed-meshheading:8380300-Tumor Suppressor Proteins
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pubmed:year |
1993
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pubmed:articleTitle |
Localization of the chromosome 15 breakpoints and expression of multiple PML-RAR alpha transcripts in acute promyelocytic leukemia: a study of 28 Chinese patients.
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pubmed:affiliation |
Laboratory of Molecular Biology, Rui-Jin Hospital, Shanghai Second Medical University, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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