Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-1-29
pubmed:abstractText
Anthrax lethal toxin, which consists of two proteins, protective antigen and lethal factor, is cytolytic for macrophages. Macrophages from different mouse strains were found to vary in their sensitivities to toxin. C3H mouse macrophages lysed by lethal factor concentrations of 0.001 micrograms/ml were 100,000 times more sensitive than those from resistant A/J mice. We analyzed various stages of the intoxication process to determine the basis for this resistance. Direct binding studies with radioiodinated protective antigen revealed that the affinity (Kd, approximately 0.5 nM) and number of receptors per cell (25,000 to 33,000) were the same in sensitive and resistant cells. Proteolytic activation of protective antigen by a cell surface protease and subsequent binding of lethal factor were also the same in both sensitive and resistant macrophages. Resistant A/J macrophages were not cross-resistant to other toxins and a virus which, like lethal toxin, require vesicular acidification for activity, implying that resistance is not due to a defect in vesicular acidification. When introduced into the cytosol by osmotic lysis of pinosomes, lethal factor in the absence of protective antigen was cytolytic for the sensitive macrophages while resistant cells were unaffected. Thus, lethal factor by itself possesses the toxic activity of lethal toxin. These results suggest that macrophage resistance is due to a defect at a stage occurring after toxin internalization. A/J macrophages may lack the putative lethal factor target in the cytosol or be defective in the further processing or activation of lethal factor in the cytosol or in endocytic vesicles.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-11187, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-13866126, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-13916257, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-1787799, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-1848556, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-1909998, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-1968060, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2298808, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2429952, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2499545, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2500434, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2509473, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2525560, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2554887, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2568890, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2760027, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2882752, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2895741, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2900073, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2908634, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2925786, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2946705, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-2981852, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3033081, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3081444, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3104336, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3112141, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3143893, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3148094, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3257214, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3257460, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3283131, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3372597, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3490969, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3695968, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3711080, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3733757, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3768957, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-378423, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3876284, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-3890838, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-6254391, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-6285339, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-6321492, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-6370236, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-6411625, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-6480694, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-6501408, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-6504048, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-6577430, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-6699408, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-6703488, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-7391060, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-7462324, http://linkedlifedata.com/resource/pubmed/commentcorrection/8380282-7462326
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-52
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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