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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1993-10-21
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pubmed:abstractText |
We have established optimal culture conditions for growing well-differentiated atrial myocytes from newborn rats. These myocytes show the morphological features of myocytes in intact atria; including perinuclear endocrine storage granules, contractile apparatus and numerous mitochondria. The cells synthesise, store and secrete immunoreactive ANP. Stored ANP is of similar molecular weight to pro-ANP (1-126); secreted ANP is the active form ANP (99-126) but processing appears to take place slowly after secretion. ANP secretion is stimulated by endothelin. Synthesis and secretion of ANP are markedly reduced by the beta-adrenergic agonist isoproterenol, and by forskolin.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-2828
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
509-18
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8377212-Animals,
pubmed-meshheading:8377212-Atrial Function,
pubmed-meshheading:8377212-Atrial Natriuretic Factor,
pubmed-meshheading:8377212-Blood Physiological Phenomena,
pubmed-meshheading:8377212-Cell Differentiation,
pubmed-meshheading:8377212-Cell Extracts,
pubmed-meshheading:8377212-Cells, Cultured,
pubmed-meshheading:8377212-Culture Media,
pubmed-meshheading:8377212-Dexamethasone,
pubmed-meshheading:8377212-Heart Atria,
pubmed-meshheading:8377212-Hormones,
pubmed-meshheading:8377212-Isoproterenol,
pubmed-meshheading:8377212-Microscopy, Electron,
pubmed-meshheading:8377212-Radioimmunoassay,
pubmed-meshheading:8377212-Rats,
pubmed-meshheading:8377212-Rats, Wistar,
pubmed-meshheading:8377212-Time Factors
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pubmed:year |
1993
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pubmed:articleTitle |
Hormonal control of atrial natriuretic peptide synthesis and secretion from cultured atrial myocytes.
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pubmed:affiliation |
Department of Biochemistry, University of Glasgow, Scotland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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