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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
27
|
| pubmed:dateCreated |
1993-10-20
|
| pubmed:databankReference | |
| pubmed:abstractText |
The organization, sequence, and transcriptional regulation of expression of the murine S100 beta gene are reported. The gene is approximately 9 kilobase pairs in length and is composed of three exons and two introns. The deduced murine S100 beta protein sequence differs from the human S100 beta protein by only 1 amino acid. The murine S100 beta gene contains a TATA box (AATAA) and a reverse CCAAT box (ATTGG) located at 30 nucleotides and 92 nucleotides upstream of the cap site, respectively. A 149-base pair DNA fragment (-157/-9) spanning the TATA box and the reverse CCAAT box functions as a promoter. The murine S100 beta promoter drives a 4-fold higher level of transcription in glial (C6) than in non-glial (3T3) cells, suggesting the existence of a potential cell type-specific regulatory element within the promoter region. The 5'-flanking region suppresses transcription from the homologous S100 beta as well as the heterologous SV40 promoters in an orientation-independent fashion. However, the 5'-flanking region exhibits cell type specificity when suppressing the S100 beta promoter-dependent transcription, indicating its involvement in the cell type-specific expression of S100 beta gene. In order to map cell type-specific regulatory elements, transcription analyses of various deletions of the 5'-region were carried out in C6 and 3T3 cells. Two cell type-specific negative regulatory elements, one active in non-glial cells and another active in glial cells, were mapped to the regions -1552/-1234 and -1234/-551, respectively. A strong negative regulatory element and a relatively weak negative element were located in the regions -551/-157 and -1669/-1552, respectively. The murine S100 beta gene is under complex transcriptional regulation involving tonic negative control exerted by combination of multiple cis-acting regulatory elements including cell type-specific elements.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/S-100 calcium-binding protein beta...,
http://linkedlifedata.com/resource/pubmed/chemical/S100 Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
268
|
| pubmed:geneSymbol |
S100&bgr;
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
20502-11
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8376406-Amino Acid Sequence,
pubmed-meshheading:8376406-Animals,
pubmed-meshheading:8376406-Base Sequence,
pubmed-meshheading:8376406-Calcium-Binding Proteins,
pubmed-meshheading:8376406-Cattle,
pubmed-meshheading:8376406-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:8376406-Cloning, Molecular,
pubmed-meshheading:8376406-Gene Expression,
pubmed-meshheading:8376406-Gene Expression Regulation,
pubmed-meshheading:8376406-Genomic Library,
pubmed-meshheading:8376406-Humans,
pubmed-meshheading:8376406-Mice,
pubmed-meshheading:8376406-Mice, Inbred BALB C,
pubmed-meshheading:8376406-Molecular Sequence Data,
pubmed-meshheading:8376406-Nerve Growth Factors,
pubmed-meshheading:8376406-Oligodeoxyribonucleotides,
pubmed-meshheading:8376406-Rats,
pubmed-meshheading:8376406-Recombinant Fusion Proteins,
pubmed-meshheading:8376406-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8376406-Restriction Mapping,
pubmed-meshheading:8376406-S100 Proteins,
pubmed-meshheading:8376406-Sequence Homology, Amino Acid,
pubmed-meshheading:8376406-Swine,
pubmed-meshheading:8376406-Transcription, Genetic
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Organization, sequence, and expression of the murine S100 beta gene. Transcriptional regulation by cell type-specific cis-acting regulatory elements.
|
| pubmed:affiliation |
Department of Neurology, University of Maryland School of Medicine, Baltimore.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|