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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1977-4-28
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pubmed:abstractText |
Incorporation of hypoxanthine, resistance to 8-azaguanine and activation by lyophilisation have been studied in cultured human fibroblasts. Cells from one family where there was a boy with Lesch-Nyham syndrome, from two families with variant H-PRT mutations and three cell strains from patients with the Lesch-Nyham syndrome were investigated. Cells from patients with the Lesch-Nyham syndrome showed almost no hypoxanthine incorporation and resistance to concentrations of 8-azaguanine up to 10(-3) M, whereas cells of patients with partial H-PRT deficiency demonstrated variant patterns of hypoxanthine uptake and partial resistance to 8-azaguanine. Lyophilisation of fibroblast sediment from patients with the Lesch-Nyhan syndrome and patients with variant H-PRT mutations showed activation of the deficient or partially deficient H-PRT enzyme. No such activation was observed in healthy controls. Activation of lyophilised fibroblast extract from patients and controls was not obtained. These results suggest that H-PRT could be associated with the cell membranes.
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pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
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pubmed:issn |
0009-9163
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
11
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
193-200
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:837570-Azaguanine,
pubmed-meshheading:837570-Cells, Cultured,
pubmed-meshheading:837570-Female,
pubmed-meshheading:837570-Fibroblasts,
pubmed-meshheading:837570-Freeze Drying,
pubmed-meshheading:837570-Hot Temperature,
pubmed-meshheading:837570-Humans,
pubmed-meshheading:837570-Hypoxanthine Phosphoribosyltransferase,
pubmed-meshheading:837570-Hypoxanthines,
pubmed-meshheading:837570-Lesch-Nyhan Syndrome,
pubmed-meshheading:837570-Male,
pubmed-meshheading:837570-Mutation
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pubmed:year |
1977
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pubmed:articleTitle |
Genetic heterogeneity of hypoxanthine-phosphoribosyl transferase in human fibroblasts of 3 families.
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pubmed:publicationType |
Journal Article
|