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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1993-10-19
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pubmed:abstractText |
Rat liver plasma membrane alkaline phosphatase (ALP) phospho-intermediates, which have molecular masses of 151 and 135 kDa bands, were labelled at physiological pH with either (gamma-32P) ATP or 32Pi. This labeling was stabilized by a potent enzyme inhibitor, bromolevamisole (BL), and not by bromodexamisole (BD). BL augmented the rate and extent of autophosphorylation and slowed down the rate of autodephosphorylation of ALP. The phospho-intermediates labeling presented nearly the same kinetic behaviour with either (gamma-32P) ATP or 32Pi. In the presence of BL a marked decrease of the phosphorylation state of many proteins was observed in hepatocytes. BL also produced a decrease of the 32Pi uptake into hepatocytes and a decrease of the specific radioactivity of cellular ATP. BD had nearly the same effect as BL on protein phosphorylation and 32Pi uptake. These results argued against a direct involvement of ALP in Pi transport across hepatocyte plasma membrane.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0145-5680
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
469-77
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:8374502-Adenosine Triphosphate,
pubmed-meshheading:8374502-Alkaline Phosphatase,
pubmed-meshheading:8374502-Animals,
pubmed-meshheading:8374502-Biological Transport,
pubmed-meshheading:8374502-Cell Membrane,
pubmed-meshheading:8374502-Cells, Cultured,
pubmed-meshheading:8374502-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8374502-Kinetics,
pubmed-meshheading:8374502-Levamisole,
pubmed-meshheading:8374502-Liver,
pubmed-meshheading:8374502-Male,
pubmed-meshheading:8374502-Phosphates,
pubmed-meshheading:8374502-Phosphoproteins,
pubmed-meshheading:8374502-Phosphorus Radioisotopes,
pubmed-meshheading:8374502-Rats,
pubmed-meshheading:8374502-Rats, Wistar,
pubmed-meshheading:8374502-Tetramisole,
pubmed-meshheading:8374502-Time Factors
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pubmed:year |
1993
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pubmed:articleTitle |
Stabilization of phospho-intermediates of rat liver plasma membrane alkaline phosphatase by uncompetitive inhibition. Relation with phosphate uptake into hepatocytes.
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pubmed:affiliation |
Groupe de Recherche et d'Etude d' Analogues de Médicaments, Faculté de Médecine et de Pharmacie, Poitiers, France.
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pubmed:publicationType |
Journal Article
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