Linked Life Data

8371628

Source:http://linkedlifedata.com/resource/pubmed/id/8371628

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
1993-10-13
pubmed:abstractText
The apical (AP)-to-basolateral (BL) transport of [3H]biotin across Caco-2 cell monolayers grown on a Transwell polycarbonate membrane was shown to be dependent on days in culture, reaching a maximum at 10 days. The AP-to-BL transport of [3H]biotin was always much greater than the BL-to-AP transport. AP-to-BL transport of [3H]biotin was concentration-(Km = 1.49 microM and Vmax = 1.86 pmol.mg protein-1.minute-1), temperature-, Na(+)- and energy-dependent, and proceeded against a concentration gradient in the BL side. [3H]Biotin transport was inhibited by unlabeled biotin and several structural analogues (e.g., desthiobiotin and biotin methyl ester) but not by taurocholic acid. No metabolism of [3H]biotin was detected in the AP-to-BL transport across Caco-2 cell monolayers. This study demonstrates that [3H]biotin transport in Caco-2 cells occurs by a carrier-mediated process.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0024-3205
pubmed:author
pubmed:issnType
Print
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1121-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Biotin transport in a human intestinal epithelial cell line (Caco-2).
pubmed:affiliation
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66045.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't