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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1993-9-24
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pubmed:databankReference |
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pubmed:abstractText |
A cDNA library prepared from the mouse osteoblastic cell line MC3T3-E1 was screened for the presence of specifically expressed genes by employing a combined subtraction hybridization/differential screening approach. A cDNA was identified and sequenced which encodes a protein designated osteoblast-specific factor 2 (OSF-2) comprising 811 amino acids. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The protein lacks a typical transmembrane region. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin I. RNA analyses revealed that OSF-2 is expressed in bone and to a lesser extent in lung, but not in other tissues. Mouse OSF-2 cDNA was subsequently used as a probe to clone the human counterpart. Mouse and human OSF-2 show a high amino acid sequence conservation except for the signal sequence and two regions in the C-terminal domain in which 'in-frame' insertions or deletions are observed, implying alternative splicing events. On the basis of the amino acid sequence homology with fasciclin I, we suggest that OSF-2 functions as a homophilic adhesion molecule in bone formation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-1545245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-1585836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-1701634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-1733501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-1850258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2208292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2335571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2394715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2406026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2412224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2418980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2435793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2447074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2448875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2481941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2493001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2659348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2698313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2821619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-2958481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-3000756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-3024151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-3025450,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-3511940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-3714490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-4053038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-5420325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-6546423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8363580-7108955
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
294 ( Pt 1)
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pubmed:geneSymbol |
OSF-2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
271-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8363580-3T3 Cells,
pubmed-meshheading:8363580-Amino Acid Sequence,
pubmed-meshheading:8363580-Animals,
pubmed-meshheading:8363580-Base Sequence,
pubmed-meshheading:8363580-Bone and Bones,
pubmed-meshheading:8363580-Cell Adhesion Molecules,
pubmed-meshheading:8363580-Cell Adhesion Molecules, Neuronal,
pubmed-meshheading:8363580-Cell Line,
pubmed-meshheading:8363580-Cloning, Molecular,
pubmed-meshheading:8363580-DNA,
pubmed-meshheading:8363580-Gene Expression Regulation,
pubmed-meshheading:8363580-Humans,
pubmed-meshheading:8363580-Insects,
pubmed-meshheading:8363580-Mice,
pubmed-meshheading:8363580-Molecular Sequence Data,
pubmed-meshheading:8363580-RNA, Messenger,
pubmed-meshheading:8363580-Sequence Homology, Amino Acid
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pubmed:year |
1993
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pubmed:articleTitle |
Osteoblast-specific factor 2: cloning of a putative bone adhesion protein with homology with the insect protein fasciclin I.
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