Linked Life Data

8360939

Source:http://linkedlifedata.com/resource/pubmed/id/8360939

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-9-30
pubmed:abstractText
Guinea pigs have been used extensively to model pulmonary hypersensitivity responses. Although guinea pigs produce mainly immunoglobulin G1 (IgG1) antibodies and humans produce IgE, both immunoglobulin classes have been shown to be regulated similarly. We used an established guinea pig model to examine the role of IgG1 in immediate- and late-onset pulmonary hypersensitivity responses. IgG1 was purified from the serum of ovalbumin-immunized animals and shown to be free of IgE. It was transferred into naive recipients in doses quantified on the basis of its biological activity as measured in the passive cutaneous anaphylaxis (PCA) assay. Inhalation challenge of recipient animals 24 h later with an ovalbumin aerosol produced immediate-onset airway constrictive responses, with response dependent upon the quantity of antibody passively administered. None of the recipient animals displayed a late-phase response previously shown to be characterized by increased breathing frequency, airflow limitation during exhalation, and mild fever. However, pulmonary eosinophilia, measured at 24 h postinhalation challenge, was detected with the severity of the eosinophilia dependent upon the quantity of IgG1 administered. The results indicated that immediate-, but not late-onset, responses were associated with IgG1 antibody. Occurrence of late-onset pulmonary eosinophilia indicated that eosinophilic inflammation, a recognized characteristic of hypersensitivity responses, was related to antigen-specific IgG1 antibody.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0098-4108
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
117-27
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Role of purified IgG1 in pulmonary hypersensitivity responses of the guinea pig.
pubmed:affiliation
Department of Environmental and Occupational Health, Graduate School of Public Health, University of Pittsburgh, PA 15238.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.