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rdf:type |
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lifeskim:mentions |
umls-concept:C0021017,
umls-concept:C0025255,
umls-concept:C0034818,
umls-concept:C0205349,
umls-concept:C0242692,
umls-concept:C0332307,
umls-concept:C0449774,
umls-concept:C0681850,
umls-concept:C1550501,
umls-concept:C1706203,
umls-concept:C2349001,
umls-concept:C2697811
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pubmed:issue |
7
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pubmed:dateCreated |
1993-9-24
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pubmed:abstractText |
The human insulin receptor exists in two isoforms (HIR-A alpha-subunit 719 amino acids and HIR-B alpha-subunit 731 amino acids) which are generated by alternative splicing of a small exon and display distinct patterns of tissue-specific expression. Using the polymerase chain reaction we have recently shown that skeletal muscle of non-diabetic individuals contains predominantly mRNA encoding HIR-A while in skeletal muscle derived from subjects with Type 2 (non-insulin-dependent) diabetes mellitus similar amounts of each mRNA are expressed. We used a polyclonal antibody which discriminates between HIR-A and HIR-B to assess the isoform expression at the protein level. The antibody showed clearly distinct displacement of insulin binding in skeletal muscle membranes of non-diabetic subjects compared to Type 2 diabetic subjects (displacement of specific 125I-insulin binding: 13 non-diabetic subjects 70.0% +/- 14.34, 12 Type 2 diabetic subjects 32.6% +/- 17.45). A control antibody which does not discriminate between both isoforms showed similar displacement of 125I-insulin in membranes of non-diabetic and Type 2 diabetic subjects. These data suggest that the altered expression of receptor isotype mRNA in the skeletal muscle of Type 2 diabetic subjects leads to an altered receptor isoform pattern in the plasma membrane. While skeletal muscle membranes of non-diabetic subjects contain predominantly HIR-A, membranes of Type 2 diabetic subjects show an increased level of HIR-B in addition to HIR-A.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Blood Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Hemoglobin A, Glycosylated,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/L-Lactate Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphofructokinase-1,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoglucomutase,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoglycerate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Insulin
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0012-186X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
628-32
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8359580-Aged,
pubmed-meshheading:8359580-Aged, 80 and over,
pubmed-meshheading:8359580-Alternative Splicing,
pubmed-meshheading:8359580-Animals,
pubmed-meshheading:8359580-Blood Glucose,
pubmed-meshheading:8359580-Cell Line,
pubmed-meshheading:8359580-Diabetes Mellitus, Type 2,
pubmed-meshheading:8359580-Hemoglobin A, Glycosylated,
pubmed-meshheading:8359580-Humans,
pubmed-meshheading:8359580-Insulin,
pubmed-meshheading:8359580-Kinetics,
pubmed-meshheading:8359580-L-Lactate Dehydrogenase,
pubmed-meshheading:8359580-Macromolecular Substances,
pubmed-meshheading:8359580-Middle Aged,
pubmed-meshheading:8359580-Muscles,
pubmed-meshheading:8359580-Phosphofructokinase-1,
pubmed-meshheading:8359580-Phosphoglucomutase,
pubmed-meshheading:8359580-Phosphoglycerate Kinase,
pubmed-meshheading:8359580-RNA, Messenger,
pubmed-meshheading:8359580-Rats,
pubmed-meshheading:8359580-Receptor, Insulin,
pubmed-meshheading:8359580-Reference Values,
pubmed-meshheading:8359580-Transfection
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pubmed:year |
1993
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pubmed:articleTitle |
Altered pattern of insulin receptor isotypes in skeletal muscle membranes of type 2 (non-insulin-dependent) diabetic subjects.
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pubmed:affiliation |
Institut für Diabetesforschung, Munich, Germany.
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pubmed:publicationType |
Journal Article
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