Linked Life Data

8359221

Source:http://linkedlifedata.com/resource/pubmed/id/8359221

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-9-30
pubmed:abstractText
A nonradioactive ultrastructural method based on the incorporation of 5-bromouridine-5'-triphosphate into the RNA of streptolysin O-permeabilized cultured HeLa cells is described and used for the visualization of rRNA transcription sites. Even though the method provides much better resolution than ultrastructural autoradiography, the results obtained do not allow the assignment of rRNA transcription to a single nucleolar structural component. We locate the rRNA transcription sites at the border region of fibrillar centers with dense fibrillar components. In addition, the method represents a convenient tool for the in situ immunodetection of extranucleolar RNA synthesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:volume
208
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
275-81
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Nonisotopic ultrastructural mapping of transcription sites within the nucleolus.
pubmed:affiliation
Institute of Experimental Medicine, Academy of Sciences of Czech Republic, Prague.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't