Linked Life Data

8358865

Source:http://linkedlifedata.com/resource/pubmed/id/8358865

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-9-24
pubmed:abstractText
The insulin-like growth factors I and II (IGF I and II) and their cell surface receptors are expressed in the mammalian embryo and may function as autocrine or paracrine growth factors during early development. P19 embryonic carcinoma cells, derived from a 7.5 day mouse embryo, were used as a model for a functional study of the IGF system in post-implantation embryogenesis. Undifferentiated P19 cells synthesized IGF I and II, the type I and II IGF receptors, and IGF binding proteins (IGF BP2, IGF BP3, and IGF BP4). P19 cells showed an increase in thymidine incorporation of 150% of control with a 4 hour incubation of IGF I (10 ng/ml) or IGF II (100 ng/ml) and an increase in cell viability compared to control cells during 24 hours of serum starvation. In both experiments IGF I was more potent than IGF II. Endogenous concentrations of IGF I and II in conditioned media were low compared to the doses of exogenous IGFs required for biologic effect, but nonetheless contributed significantly to baseline DNA synthesis, as demonstrated by inhibition of IGF actions with specific antibodies. Cell surface associated IGF BPs bound more radiolabeled IGF than IGF receptors, as determined by binding studies and affinity cross-linking. IGF I and IGF II appeared to regulate production of IGF BP2, suggesting that the IGFs may regulate their own actions by altering the abundance of their binding proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0192-253X
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
194-203
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8358865-Animals, pubmed-meshheading:8358865-Cell Differentiation, pubmed-meshheading:8358865-Cell Division, pubmed-meshheading:8358865-Cell Line, pubmed-meshheading:8358865-Cell Survival, pubmed-meshheading:8358865-DNA, pubmed-meshheading:8358865-DNA Probes, pubmed-meshheading:8358865-DNA Replication, pubmed-meshheading:8358865-Embryo, Mammalian, pubmed-meshheading:8358865-Insulin-Like Growth Factor I, pubmed-meshheading:8358865-Insulin-Like Growth Factor II, pubmed-meshheading:8358865-Mice, pubmed-meshheading:8358865-Oligodeoxyribonucleotides, pubmed-meshheading:8358865-Polymerase Chain Reaction, pubmed-meshheading:8358865-RNA, Messenger, pubmed-meshheading:8358865-Receptor, IGF Type 1, pubmed-meshheading:8358865-Receptor, IGF Type 2, pubmed-meshheading:8358865-Teratoma, pubmed-meshheading:8358865-Thymidine, pubmed-meshheading:8358865-Tritium
pubmed:year
1993
pubmed:articleTitle
Possible autocrine/paracrine actions of insulin-like growth factors during embryonic development: expression and action of IGFs in undifferentiated P19 cells.
pubmed:affiliation
Department of Pediatrics, University of North Carolina, Chapel Hill.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.