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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1993-9-27
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pubmed:databankReference |
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pubmed:abstractText |
A cDNA coding for glucoamylase P of Hormoconis resinae was cloned using a synthetic oligonucleotide probe coding for a peptide fragment of the purified enzyme and polyclonal anti-glucoamylase antibodies. Nucleotide-sequence analysis revealed an open reading frame of 1848 base pairs coding for a protein of 616 amino-acid residues. Comparison with other fungal glucoamylase amino-acid sequences showed homologies of 37-48%. The glucoamylase cDNA, when introduced into Saccharomyces cerevisiae under the control of the yeast ADC1 promoter, directed the secretion of active glucoamylase P into the growth medium.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0172-8083
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
38-44
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pubmed:dateRevised |
2000-12-18
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pubmed:meshHeading |
pubmed-meshheading:8358830-Amino Acid Sequence,
pubmed-meshheading:8358830-Base Sequence,
pubmed-meshheading:8358830-Blotting, Western,
pubmed-meshheading:8358830-Cladosporium,
pubmed-meshheading:8358830-Cloning, Molecular,
pubmed-meshheading:8358830-DNA, Fungal,
pubmed-meshheading:8358830-Gene Library,
pubmed-meshheading:8358830-Glucan 1,4-alpha-Glucosidase,
pubmed-meshheading:8358830-Molecular Sequence Data,
pubmed-meshheading:8358830-Restriction Mapping,
pubmed-meshheading:8358830-Saccharomyces cerevisiae
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pubmed:articleTitle |
Cloning and expression of Hormoconis resinae glucoamylase P cDNA in Saccharomyces cerevisiae.
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pubmed:affiliation |
Research Laboratories, Alko Ltd, Helsinki, Finland.
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pubmed:publicationType |
Journal Article
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