Linked Life Data

8356113

Source:http://linkedlifedata.com/resource/pubmed/id/8356113

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-9-21
pubmed:abstractText
The 8.6-kb kalilo linear mitochondrial plasmid of Neurospora intermedia was found to give rise to multiple transcripts of 8.6, 4.4, 4.0, 1.3, 1.2, and 0.9 kb. Plasmid transcripts were found in all the developmental stages tested and were present in cells with and without plasmid integration into mtDNA. The 4.4- and 4.0-kb transcripts corresponded to the two known ORFs in size, position, and polarity. An analysis of RNA fractions has confirmed the mitochondrial location of kalilo. The kalilo-specific RNA appears to be selectively unstable in affected strains of N. intermedia; this may be a general property of linear plasmid RNA. Transcripts are most likely initiated from near the termini of the linear plasmid. Primer extension and sequencing showed a clear 5' RNA end at nucleotide 101. However, the sequence in this region does not show homology with any known mitochondrial, plasmid, or nuclear promoter. The sequence does show homology with the tip of the plasmid and marks the border of a long series of direct repeats. Possibly the plasmid RNA polymerase may be bifunctional, recognizing sequences at the plasmid tip as well as the promoter.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0147-619X
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
180-92
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Transcription of the kalilo linear senescence plasmid from Neurospora intermedia.
pubmed:affiliation
Department of Botany, University of British Columbia, Vancouver, Canada.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't