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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
23
|
| pubmed:dateCreated |
1993-9-15
|
| pubmed:databankReference | |
| pubmed:abstractText |
hXBP-1 is a transcription factor of the leucine zipper (b-zip) family important in the expression of the class II major histocompatibility complex gene, DRA. Studies with mouse-human hybrids have mapped hXBP-1 hybridizing fragments to human chromosomes 5 and 22 and the frequent detection of two mRNA transcripts suggested that hXBP-1 may be a member of a small gene family. To analyze the structure and regulation of hXBP-1 further, cosmid clones from both chromosomes were isolated. Mapping and sequence analyses reveal that chromosome 22 contains the functional hXBP-1 gene while chromosome 5 contains a processed pseudogene. hXBP-1 promoter analysis has revealed that cis-active elements within the 5'-untranslated region of hXBP-1 are essential for full promoter activity. One such element, hX2, is identical to the hXBP-1 target sequence in the DRA promoter. Mutagenesis of the hX2 site substantially decreases promoter activity. This element interacts with four distinct protein complexes in mature B cells and cross-competition experiments show that two of these complexes (complex 1 and complex 4) also interact with the hXBP-1 target sequence (X2) from the DRA promoter. The similarities of the hXBP-1 promoter and of the DRA promoter (the gene that the hXBP-1 protein regulates) are further emphasized by the fact that a Y box element is located 3' of both hX2 and X2.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR alpha-Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/regulatory factor X transcription...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
268
|
| pubmed:geneSymbol |
hXBP-1
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
17074-82
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8349596-Amino Acid Sequence,
pubmed-meshheading:8349596-Base Sequence,
pubmed-meshheading:8349596-Blotting, Southern,
pubmed-meshheading:8349596-Chromosomes, Human, Pair 22,
pubmed-meshheading:8349596-Cloning, Molecular,
pubmed-meshheading:8349596-Cosmids,
pubmed-meshheading:8349596-DNA,
pubmed-meshheading:8349596-DNA-Binding Proteins,
pubmed-meshheading:8349596-Genes, Regulator,
pubmed-meshheading:8349596-HLA-DR Antigens,
pubmed-meshheading:8349596-HLA-DR alpha-Chains,
pubmed-meshheading:8349596-HeLa Cells,
pubmed-meshheading:8349596-Humans,
pubmed-meshheading:8349596-Molecular Sequence Data,
pubmed-meshheading:8349596-Polymerase Chain Reaction,
pubmed-meshheading:8349596-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8349596-Restriction Mapping,
pubmed-meshheading:8349596-Structure-Activity Relationship,
pubmed-meshheading:8349596-Transcription, Genetic,
pubmed-meshheading:8349596-Transcription Factors
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
The regulatory gene, hXBP-1, and its target, HLA-DRA, utilize both common and distinct regulatory elements and protein complexes.
|
| pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|