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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1993-9-13
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pubmed:abstractText |
A method for typing Haemophilus species is described, based on the analysis of genomic DNA from Haemophilus parainfluenzae. The DNA was extracted by a rapid method and digested with the restriction enzyme BamHI to provide a characteristic 'fingerprint'. The pattern of fragments in the ranges 1-1.6 kb, 1.6-2 kb and 2-3 kb were used to produce a numerical profile of each isolate. In total 97 isolates were examined; 88 from throat swab material isolated from the 15 members of a British Antarctic Survey base and 9 type strains. Seventy-two of the 88 antarctic isolates were H. parainfluenzae and were found to be very diverse, comprising 41 identifiable strains with up to 5 strains being isolated from a single throat swab sample. There was evidence for both carriage and transmission within the isolated community. The technique provided a highly discriminatory method for characterizing Haemophilus strains which is suitable for epidemiological studies.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-1066093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-1548238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-1660917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-1706727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-1752303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-1967849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-2030503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-2313130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-2677160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-2687313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-2788138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-2994802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-338572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-3486294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-3487508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-3488370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-3500012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-3522641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-4566182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-5505020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-6332018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-6376625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-6816135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-7037535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-7463464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8348936-772168
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0950-2688
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
111
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
89-98
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:8348936-Adult,
pubmed-meshheading:8348936-Antarctic Regions,
pubmed-meshheading:8348936-Bacterial Typing Techniques,
pubmed-meshheading:8348936-DNA, Bacterial,
pubmed-meshheading:8348936-DNA Fingerprinting,
pubmed-meshheading:8348936-Deoxyribonuclease BamHI,
pubmed-meshheading:8348936-Electrophoresis, Agar Gel,
pubmed-meshheading:8348936-Genetic Variation,
pubmed-meshheading:8348936-Haemophilus,
pubmed-meshheading:8348936-Humans,
pubmed-meshheading:8348936-Male,
pubmed-meshheading:8348936-Respiratory System
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pubmed:year |
1993
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pubmed:articleTitle |
An analysis of the diversity of Haemophilus parainfluenzae in the adult human respiratory tract by genomic DNA fingerprinting.
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pubmed:affiliation |
British Antarctic Survey Medical Unit, Robert Gordons Institute of Technology Survival Centre, Aberdeen.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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