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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1993-9-8
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pubmed:abstractText |
In order to study the in-vitro mechanism of action of gonadotrophin surge-attenuating factor (GnSAF), ovine pituitary monolayers were incubated for 48 h either with steroid-free follicular fluid from women in whom ovulation had been induced or oestradiol or progesterone. Monolayers were also incubated with 10-30 and > 30 kDa follicular fluid fractions. The effect of these preparations on gonadotrophin-releasing hormone (GnRH)-induced luteinizing hormone (LH) secretion was then determined by incubation with GnRH for between 15 and 240 min. Incubation with steroid-free follicular fluid resulted in the significant attenuation of both the acutely releasable LH pool (LH 54.3 +/- 3.0% of control secretion at 15 min of GnRH incubation) and the reserve LH pool (LH 36.8 +/- 1.6% of control secretion at 240 min GnRH incubation). The 10-30 kDa follicular fluid fraction had similar effects while the > 30 kDa follicular fluid fraction significantly stimulated GnRH-induced LH secretion, with concentrations up to 134.0 +/- 8.6% of controls. Oestradiol or progesterone at doses of 1000 nmol/l/well did not significantly reduce any aspect of GnRH-induced LH secretion (never < 95.1 +/- 15.1% of controls). These results demonstrate that GnSAF markedly attenuates both the acutely releasable and reserve pools of GnRH-induced LH secretion. Inhibin, oestradiol and progesterone are not responsible for the marked attenuation of either the initial or reserve pools of GnRH-induced LH secretion.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Gonadal Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Gonadotropin-Releasing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Inhibins,
http://linkedlifedata.com/resource/pubmed/chemical/Luteinizing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Progesterone,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/attenuin
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0268-1161
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
822-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8345069-Animals,
pubmed-meshheading:8345069-Basal Metabolism,
pubmed-meshheading:8345069-Blood Physiological Phenomena,
pubmed-meshheading:8345069-Cells, Cultured,
pubmed-meshheading:8345069-Estradiol,
pubmed-meshheading:8345069-Female,
pubmed-meshheading:8345069-Follicular Fluid,
pubmed-meshheading:8345069-Gonadal Hormones,
pubmed-meshheading:8345069-Gonadotropin-Releasing Hormone,
pubmed-meshheading:8345069-Humans,
pubmed-meshheading:8345069-Inhibins,
pubmed-meshheading:8345069-Luteinizing Hormone,
pubmed-meshheading:8345069-Male,
pubmed-meshheading:8345069-Molecular Weight,
pubmed-meshheading:8345069-Ovulation Induction,
pubmed-meshheading:8345069-Pituitary Gland,
pubmed-meshheading:8345069-Progesterone,
pubmed-meshheading:8345069-Proteins,
pubmed-meshheading:8345069-Sheep
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pubmed:year |
1993
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pubmed:articleTitle |
Effects of gonadotrophin surge-attenuating factor on the two pools of gonadotrophin-releasing hormone-induced luteinizing hormone secretion in vitro.
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pubmed:affiliation |
Department of Obstetrics and Gynaecology, University of Aberdeen, Foresterhill, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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