Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1993-9-8
pubmed:abstractText
In order to study the in-vitro mechanism of action of gonadotrophin surge-attenuating factor (GnSAF), ovine pituitary monolayers were incubated for 48 h either with steroid-free follicular fluid from women in whom ovulation had been induced or oestradiol or progesterone. Monolayers were also incubated with 10-30 and > 30 kDa follicular fluid fractions. The effect of these preparations on gonadotrophin-releasing hormone (GnRH)-induced luteinizing hormone (LH) secretion was then determined by incubation with GnRH for between 15 and 240 min. Incubation with steroid-free follicular fluid resulted in the significant attenuation of both the acutely releasable LH pool (LH 54.3 +/- 3.0% of control secretion at 15 min of GnRH incubation) and the reserve LH pool (LH 36.8 +/- 1.6% of control secretion at 240 min GnRH incubation). The 10-30 kDa follicular fluid fraction had similar effects while the > 30 kDa follicular fluid fraction significantly stimulated GnRH-induced LH secretion, with concentrations up to 134.0 +/- 8.6% of controls. Oestradiol or progesterone at doses of 1000 nmol/l/well did not significantly reduce any aspect of GnRH-induced LH secretion (never < 95.1 +/- 15.1% of controls). These results demonstrate that GnSAF markedly attenuates both the acutely releasable and reserve pools of GnRH-induced LH secretion. Inhibin, oestradiol and progesterone are not responsible for the marked attenuation of either the initial or reserve pools of GnRH-induced LH secretion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0268-1161
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
822-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8345069-Animals, pubmed-meshheading:8345069-Basal Metabolism, pubmed-meshheading:8345069-Blood Physiological Phenomena, pubmed-meshheading:8345069-Cells, Cultured, pubmed-meshheading:8345069-Estradiol, pubmed-meshheading:8345069-Female, pubmed-meshheading:8345069-Follicular Fluid, pubmed-meshheading:8345069-Gonadal Hormones, pubmed-meshheading:8345069-Gonadotropin-Releasing Hormone, pubmed-meshheading:8345069-Humans, pubmed-meshheading:8345069-Inhibins, pubmed-meshheading:8345069-Luteinizing Hormone, pubmed-meshheading:8345069-Male, pubmed-meshheading:8345069-Molecular Weight, pubmed-meshheading:8345069-Ovulation Induction, pubmed-meshheading:8345069-Pituitary Gland, pubmed-meshheading:8345069-Progesterone, pubmed-meshheading:8345069-Proteins, pubmed-meshheading:8345069-Sheep
pubmed:year
1993
pubmed:articleTitle
Effects of gonadotrophin surge-attenuating factor on the two pools of gonadotrophin-releasing hormone-induced luteinizing hormone secretion in vitro.
pubmed:affiliation
Department of Obstetrics and Gynaecology, University of Aberdeen, Foresterhill, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't