Linked Life Data

8344517

Source:http://linkedlifedata.com/resource/pubmed/id/8344517

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-9-3
pubmed:databankReference
pubmed:abstractText
The whiE gene cluster of Streptomyces coelicolor, which is related to gene sets encoding the biosynthesis of polycyclic aromatic polyketide antibiotics, determines a spore pigment. Southern blotting using probes from three different parts of the whiE cluster revealed related gene sets in about half of a collection of diverse Streptomyces strains. A 5.2-kb segment of one such cluster, sch, previously shown to determine spore pigmentation in Streptomyces halstedii, was sequenced. Seven open reading frames (ORFs), two of them incomplete, were found. Six of the ORFs resemble the known part of the whiE cluster closely. The derived gene products include a ketosynthase (= condensing enzyme) pair, acyl carrier protein and cyclase, as well as two of unidentified function. The seventh ORF diverges from the main cluster and encodes a protein that resembles a dichlorophenol hydroxylase. Comparison with sequences of related gene sets for the biosynthesis of antibiotics suggests that gene clusters destined to specify pigment production diverged from those destined to specify antibiotics early in the evolution of the Streptomyces genus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
130
pubmed:geneSymbol
sch, whiE
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
107-16
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:8344517-Amino Acid Sequence, pubmed-meshheading:8344517-Anti-Bacterial Agents, pubmed-meshheading:8344517-Bacterial Proteins, pubmed-meshheading:8344517-Base Sequence, pubmed-meshheading:8344517-Biological Evolution, pubmed-meshheading:8344517-Blotting, Southern, pubmed-meshheading:8344517-Carrier Proteins, pubmed-meshheading:8344517-Conserved Sequence, pubmed-meshheading:8344517-DNA, Bacterial, pubmed-meshheading:8344517-DNA Probes, pubmed-meshheading:8344517-Fatty Acids, pubmed-meshheading:8344517-Genes, Bacterial, pubmed-meshheading:8344517-Molecular Sequence Data, pubmed-meshheading:8344517-Multienzyme Complexes, pubmed-meshheading:8344517-Multigene Family, pubmed-meshheading:8344517-Open Reading Frames, pubmed-meshheading:8344517-Oxidoreductases, pubmed-meshheading:8344517-Peptides, pubmed-meshheading:8344517-Pigments, Biological, pubmed-meshheading:8344517-Sequence Alignment, pubmed-meshheading:8344517-Sequence Analysis, DNA, pubmed-meshheading:8344517-Sequence Homology, Amino Acid, pubmed-meshheading:8344517-Sequence Homology, Nucleic Acid, pubmed-meshheading:8344517-Spores, Bacterial, pubmed-meshheading:8344517-Streptococcus, pubmed-meshheading:8344517-beta-Glucosidase
pubmed:year
1993
pubmed:articleTitle
Hybridization and DNA sequence analyses suggest an early evolutionary divergence of related biosynthetic gene sets encoding polyketide antibiotics and spore pigments in Streptomyces spp.
pubmed:affiliation
Departamento de Biología Funcional, Universidad de Oviedo, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't