Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1993-9-8
pubmed:databankReference
pubmed:abstractText
To search genetically for additional components of the protein translocation apparatus of mitochondria, we have used low fidelity PCR mutagenesis to generate temperature-sensitive mutants in the outer membrane translocation pore component ISP42. A high copy number suppressor of temperature-sensitive isp42 has been isolated and sequenced. This novel gene, denoted ISP6, encodes a 61 amino acid integral membrane protein of the mitochondrial outer membrane, which is oriented with its amino-terminus facing the cytosol. Disruption of the ISP6 gene is without apparent effect in wild type yeast cells, but is lethal in temperature-sensitive isp42 mutants. Immunoprecipitation of the gene product, ISP42p, from mitochondria solubilized under mild conditions reveals a multi-protein complex containing ISP6p and ISP42p.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-1327874, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-1530986, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-16453466, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-1834661, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-2005794, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-2005804, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-2174514, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-2250717, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-2265609, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-2449095, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-2455217, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-2546682, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-2556402, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-2659436, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-2674724, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-2692852, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-2868014, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-2876995, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-3319781, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-3323810, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-3327466, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-395030, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-6290489, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-6313478, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-6424121, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-7039847, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-7108955, http://linkedlifedata.com/resource/pubmed/commentcorrection/8344244-8416924
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:geneSymbol
ISP42, ISP6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3023-34
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed-meshheading:8344244-Amino Acid Sequence, pubmed-meshheading:8344244-Base Sequence, pubmed-meshheading:8344244-DNA, Fungal, pubmed-meshheading:8344244-Fungal Proteins, pubmed-meshheading:8344244-Genes, Suppressor, pubmed-meshheading:8344244-Hot Temperature, pubmed-meshheading:8344244-Intracellular Membranes, pubmed-meshheading:8344244-Macromolecular Substances, pubmed-meshheading:8344244-Membrane Proteins, pubmed-meshheading:8344244-Membrane Transport Proteins, pubmed-meshheading:8344244-Mitochondria, pubmed-meshheading:8344244-Mitochondrial Membrane Transport Proteins, pubmed-meshheading:8344244-Molecular Sequence Data, pubmed-meshheading:8344244-Polymerase Chain Reaction, pubmed-meshheading:8344244-Saccharomyces cerevisiae, pubmed-meshheading:8344244-Saccharomyces cerevisiae Proteins
pubmed:year
1993
pubmed:articleTitle
Genetic and biochemical characterization of ISP6, a small mitochondrial outer membrane protein associated with the protein translocation complex.
pubmed:affiliation
Department of Biochemistry and Biophysics, University of North Carolina, School of Medicine, Chapel Hill 27599-7260.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.